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International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v7.i4.60
pages 565-572

Molecular Identification of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data

S. K. Singh
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India
R. D. Rai
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP
Shwet Kamal
National Research Centre for Mushroom (ICAR), Chambaghat, Solan, HP, India

要約

Analysis of genetic relationship of important Aphyllophorales germplasm accessions of the National Research Centre for Mushroom, Solan, India, was conducted using RAPD and ITS rDNA sequences. RAPD profiles exhibited significant variations among Trametes versicolor, Tyromyces chioneus, and Ganoderma species. The resulting RAPD-based phylogram grouped Ganoderma spp. accessions into four clades. Phylogeny inferred from ITS rDNA sequence distinguished Trametes versicolor, Tyromyces chioneus, and Ganoderma spp. at the generic level due to different lengths and variations of the 5.8S rDNA conserved region. At the intrageneric level, Ganoderma accessions were divided into two distinct groups based on nucleotide substitution in ITS-1 and ITS-2 regions. It is presumed that some of the Ganoderma sp. samples might be G. lucidum. Single nucleotide substitutions turned out to be more useful for genetic cataloguing of isolates within a species than sequence alignment using NCBI databse.


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