年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
In Vitro Anti-proliferative Effects on NB4 Human Leukemia Cells and Physicochemical Screening of Pleurotus sp. (Higher Basidiomycetes) Mycelia from Cuba
要約
This study examined the phytochemical profile and the in vitro anti-proliferative effects of a hot water mycelial extract from the edible mushroom Pleurotus sp. on NB4 human leukemia cells. Flow-cytometry analyses were used to measure cell viability, cell cycle, and apoptosis in cells incubated 24 h with the extract at doses of 100 and 200 µg/mL. Pleurotus sp. extract reduced cell viability, particularly at the concentration of 200 µg/mL to 82% compared to control cells, and induced apoptosis demonstrated by an increase in the number of annexin V-FITC+ cells (25% at 200 µg/mL). The NB4 cells were arrested in the G2/M phase thus supporting a cell-cycle dependent anticancer mechanism. Although carbohydrates (76.8%, w/w) appear to be the most important antitumor compound, secondary metabolites-like phenolics would also contribute to the anti-proliferative activity. The results indicate that Pleurotus sp. mycelia obtained by submerged fermentation may be an interesting renewable resource for developing functional foods and new antitumor therapeutic agents.
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Llauradó Gabriel, Morris Humberto J., Ferrera Leodán, Camacho Miladis, Castán Leniher, Lebeque Yamila, Beltrán Yaixa, Cos Paul, Bermúdez Rosa C., In-vitro antimicrobial activity and complement/macrophage stimulating effects of a hot-water extract from mycelium of the oyster mushroom Pleurotus sp., Innovative Food Science & Emerging Technologies, 30, 2015. Crossref
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Morris Humberto J., Llauradó Gabriel, Beltrán Yaixa, Lebeque Yamila, Bermúdez Rosa C., García Nora, Gaime-Perraud Isabelle, Moukha Serge, The Use of Mushrooms in the Development of Functional Foods, Drugs, and Nutraceuticals, in Wild Plants, Mushrooms and Nuts, 2016. Crossref
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Llauradó Maury Gabriel, Morris-Quevedo Humberto J., Heykers Annick, Lanckacker Ellen, Cappoen Davie, Delputte Peter, Vanden Berghe Wim, Salgueiro Zelene, Cos Paul, Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from Pleurotus ostreatus Mycelium, Journal of Fungi, 7, 3, 2021. Crossref
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Vieira Gomes Débora Cássia, de Alencar Marcus Vinícius Oliveira Barros, dos Reis Antonielly Campinho, de Lima Rosália Maria Torres, de Oliveira Santos José Victor, da Mata Ana Maria Oliveira Ferreira, Soares Dias Ana Carolina, da Costa Joaquim Soares, de Medeiros Maria das Graças Freire, Paz Márcia Fernanda Correia Jardim, Gayoso e Almendra Ibiapina Moreno Lina Clara, Castro e Sousa João Marcelo de, Islam Muhammad Torequl, Melo Cavalcante Ana Amélia de Carvalho, Antioxidant, anti-inflammatory and cytotoxic/antitumoral bioactives from the phylum Basidiomycota and their possible mechanisms of action, Biomedicine & Pharmacotherapy, 112, 2019. Crossref
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