ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i2.40
pages 121-130

In Vitro Antitumor Activity and Structure Characterization of Ethanol Extracts from Wild and Cultivated Chaga Medicinal Mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae)

Yong Sun
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Ting Yin
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Xian-Hui Chen
Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou
Gong Zhang
University of Winnipeg, Manitoba, Canada
Rempel B. Curtis
Richardson Center for Functional Foods and Nutraceuticals, University of Manitoba, Manitoba, Canada
Zhan-Hui Lu
Department of Food Science and Engineering, China Agricultural University, Beijing
Ji-Hong Jiang
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, China

要約

Inonotus obliquus (Pers.:Fr.) Pilat has been traditionally used as a folk remedy for treatment of cancers, cardiovascular disease and diabetes in Russia, Poland, and most of the Baltic countries, but natural reserves of this fungus have nearly been exhausted. This study was designed to investigate the artificial cultivation of I. obliquus and the antitumor activity of its tissues. The ethanol extract of cultivated sclerotium had the highest cell growth inhibitory rate (74.6%) as determined by an 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. 78% of the bags produced sclerotia and only 6.17 g/bag of sclerotium was obtained. Extracts of the cultivated fruiting body showed 44.2% inhibitory activity against tumor cells. However, the yield was as high as 18.24 g/bag, and 98% of the bags produced fruiting body. The results of gas chromatography-mass spectroscopy (GC-MS) showed that similar compounds were extracted from the wild and cultivated samples. The principal compounds observed were lanosterol, inotodiol, and ergosterol. Their percentages of the mass fraction were 86.1, 59.9, and 71.8% of the total, for the wild sclerotium, cultivated sclerotium, and cultivated fruiting body, respectively. Ergosterol was found to be much higher (27.32%) in cultivated fruiting body. We conclude that cultivated fruiting body of I. obliquus obtained by inoculation of the substrate with spawn mycelium of the fifth generation could serve as an ideal substitute for the wild I. obliquus.


Articles with similar content:

Production of Basidiomata and Ligninolytic Enzymes by the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Licuri (Syagrus coronata) Wastes in Brazil
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Luciana Porto de Souza Vandenberghe, Aline Simoes da Rocha Bispo, Helio Mitoshi Kamida, Maria Gabriela Bello Koblitz, Aristoteles Goes-Neto, Thais Almeida de Menezes
High-Performance Liquid Chromatography Studies to Estimate Ergosterol Content at Different Developmental Stages of the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Anu Kalia, Anna Goyal, Harpreet Singh Sodhi
Antioxidant and Antiproliferative Potential of Fruiting Bodies of the Wild-Growing King Bolete Mushroom, Boletus edulis (Agaricomycetes), from Western Serbia
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 1
Nebojsa llic, Sonja Kaisarevic, Aleksandra Novakovic, Maja Karaman, Tanja Radusin
Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian Habitats: Cultivation, Isolation, and Testing of Active Compounds
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 1
Irena Zore, Branka Wraber, Jozica Habijanic, Damjan Hodzar, Bojana Boh, Marin Berovic
Species of Genus Ganoderma (Agaricomycetes) Fermentation Broth: A Novel Antioxidant and Antimicrobial Agent
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 5
Mirjana Stajić, Jelena Vukojević, Marijana Kosanic, Jasmina Ćilerdžić, Branislav Ranković