年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus
要約
In this study, we investigated the effects of the aqueous extracts of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, obtained from three localities (China; and Morelos and Michoacan, Mexico) on cervical cells transformed by human papillomavirus (HeLa and SiHa) and C-33A cancer cells. The cells were plated in DMEM medium supplemented, and were incubated in the presence of different concentrations of G. lucidum for 24 h. Cell proliferation was determined by MTT colorimetric assay and viability by trypan blue assay. Inhibitory dose was determined (IC50) of the three different extracts of G. lucidum in the culture cell lines mentioned above. The apoptosis process was confirmed by nuclear DNA fragmentation and the cell cycle was determined by flow cytometry. The results showed that aqueous extracts G. lucidum obtained from three localities produced inhibition in the proliferation of VPH transformed cells; they also induced apoptosis and cell cycle arrest in HeLa, SiHa, and C-33A cancer cells. Therefore, it was found that aqueous extracts G. lucidum obtained from three different locations produced inhibitory effect on cancer cells and may have a potential therapeutic use for the prevention and treatment of this disease.
-
Siwulski Marek, Sobieralski Krzysztof, Golak-Siwulska Iwona, Sokół Sławomir, Sękara Agnieszka, Ganoderma lucidum (Curt.: Fr.) Karst. – health-promoting properties. A review, Herba Polonica, 61, 3, 2015. Crossref
-
Sum Simon Siu-Man, Ziegler Jane, The Undisclosed Usage of Dietary Supplements in Chinese Elderly Patients, Topics in Clinical Nutrition, 32, 4, 2017. Crossref
-
Bhardwaj Anuja, Misra Kshipra, Ganoderma sp.: The Royal Mushroom for High-Altitude Ailments, in Management of High Altitude Pathophysiology, 2018. Crossref
-
Ahmad Md Faruque, Ganoderma lucidum: Persuasive biologically active constituents and their health endorsement, Biomedicine & Pharmacotherapy, 107, 2018. Crossref
-
Fraile-Fabero Raúl, Ozcariz-Fermoselle María V., Oria-de-Rueda-Salgueiro Juan A., Garcia-Recio Veronica, Cordoba-Diaz Damian, del P. Jiménez-López María, Girbés-Juan Tomás, Differences in Antioxidants, Polyphenols, Protein Digestibility and Nutritional Profile between Ganoderma lingzhi from Industrial Crops in Asia and Ganoderma lucidum from Cultivation and Iberian Origin, Foods, 10, 8, 2021. Crossref
-
Raman Jegadeesh, Lakshmanan Hariprasath, Hyun-Jae Shin, Jang Kab-yel, The Nutritional and Pharmacological Potential of Medicinal Mushroom “Ganoderma lucidum (Lingzhi or Reishi)”, in Biology, Cultivation and Applications of Mushrooms, 2022. Crossref
-
El Sheikha Aly Farag El, Nutritional Profile and Health Benefits of Ganoderma lucidum “Lingzhi, Reishi, or Mannentake” as Functional Foods: Current Scenario and Future Perspectives, Foods, 11, 7, 2022. Crossref
-
Zhang Yu, Zhang Guoying, Ling Jianya, Medicinal Fungi with Antiviral Effect, Molecules, 27, 14, 2022. Crossref
-
Oke M. A., Afolabi F. J., Oyeleke O. O., Kilani T. A., Adeosun A. R., Olanbiwoninu A. A., Adebayo E. A., Ganoderma lucidum: Unutilized natural medicine and promising future solution to emerging diseases in Africa, Frontiers in Pharmacology, 13, 2022. Crossref