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International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i2.30
16 pages

Th1-Oriented Immunomodulating Activity of Gel-Forming Fungal (1®3)-Beta-Glucans

Naohito Ohno
Tokyo University of Pharmacy and Life Sciences School of Pharmacy, Lab Immunopharmacological and Microbiological Production, 1432-1 Horinouchi, Hachioji Tokyo, 192-0392, Japan
Takafumi Jinushi
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Toshiro Yadomae
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

要約

The immunomodulating effects of various gel-forming (1®3)-beta-glucans on balancing helper T-cell activity were examined in a murine model. Plasma from mice injected with grifolan (GRN) from the edible and medicinal mushroom Grifola frondosa (Dicks.: Fr.) S. F. Gray [or alkaline-treated sonifilan (SPG-OH)] and trinitrophenyl ovalbumin (TNP-OVA) contained TNP-specific antibodies of both IgG1 (Th2-mediated) and IgG2a (Th1-mediated) isotypes. Administration of glucan (SSG) from Sclerotinia sclerotiorum (Lib.) de Bary IFO 9395 and TNP-OVA significantly augmented the synthesis of IgG2a antibodies, while the synthesis of IgG1 was reduced. Furthermore, intracellular cytokine staining showed a higher proportion of IFN-g-producing helper T cells in SSG-administered murine splenocytes. The highest IFN-g production was observed when adherent cells of mice administered TNP-OVA and SSG were cultured with TNP-primed lymphocytes. This effect of administration of SSG on IFN-g production was completely inhibited by addition of anti-IL-12 mAb. The content of the reduced form of glutathione in splenocytes was increased by SSG administration, suggesting type 1 immunoresponse. Depletion of lymphoid dendritic cell population from SSG-administered splenocytes reduced IFN-g production. In conclusion, our study showed that beta-glucans have various effects on the Th1- or Th2-dependent antibody subclasses and, in particular, that SSG induces the development of Th1 cells via the IL-12 pathway.