ライブラリ登録: Guest
Begell Digital Portal Begellデジタルライブラリー 電子書籍 ジャーナル 参考文献と会報 リサーチ集
International Journal of Medicinal Mushrooms
インパクトファクター: 1.423 5年インパクトファクター: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344

巻:
巻 22, 2020 巻 21, 2019 巻 20, 2018 巻 19, 2017 巻 18, 2016 巻 17, 2015 巻 16, 2014 巻 15, 2013 巻 14, 2012 巻 13, 2011 巻 12, 2010 巻 11, 2009 巻 10, 2008 巻 9, 2007 巻 8, 2006 巻 7, 2005 巻 6, 2004 巻 5, 2003 巻 4, 2002 巻 3, 2001 巻 2, 2000 巻 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i8.50
pages 749-761

Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal Golden Oyster Mushroom, Pleurotus citrinopileatus (Higher Basidiomycetes)

Shin-Yi Lin
Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, Republic of China; NCHU-UCD Plant and Food Biotechnology Program, Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, Republic of China; Agricultural Biotechnology Center, NCHU, Taiwan, R.O.C
Shih-Chang Chien
The Experimental Forest Management Office, NCHU, Taichung, Taiwan, R.O.C
Sheng-Yang Wang
Department of Forestry, National Chung Hsing University, Taichung, Taiwan, Republic of China
Jeng-Leun Mau
Department of Food Science and Biotechnology, National Chung Hsing University, No. 145 Hsing-Da Road, Taichung 40227, Taiwan

要約

The optimization of submerged culture of the culinary-medicinal golden oyster mushroom, Pleurotus citrinopileatus, was studied using a one-factor-at-a-time, two-stage stimulation and central composite rotatable design to produce mycelia with high ergothioneine content. The optimal culture conditions for mycelia harvested at day 22 were a temperature of 25°C, an inoculation ratio of 5%, 2% glucose, 0.5% yeast extract, and adjustment of the initial pH value to 10. The biomass and ergothioneine content were 8.28 g/L and 10.65 mg/g dry weight (dw), respectively. The addition of an amino acid precursor increased the ergothioneine content of mycelia; cysteine was the most effective. In addition, the results obtained from central composite rotatable design showed that the recommended combination for cysteine, histidine, and methionine was 8, 4, and 0.5 mmol/L, respectively. The predicted ergothioneine content was 13.90 mg/g dw, whereas the experimental maximal ergothioneine content was 14.57 mg/g dw. With the addition of complex precursors and under optimal culture conditions, mycelia harvested at days 16−20 had higher ergothioneine content. Accordingly, the information obtained could be used to produce mycelia with high ergothioneine content.


Articles with similar content:

Isolation and Characterization of Lectins Formed by Cerrena unicolor (Higher Basidiomycetes) in Solid-State Fermentation of Sorghum and Wheat Straw
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Nona A. Mikiashvili, Vladimir I. Elisashvili
Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii (Higher Basidiomycetes) and its Composition
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Shin-Yi Lin, Jeng-Leun Mau, Ling-Yi Huang, Kung-Jui Ho, Chih-Hung Liang
Effect of Cultivation Conditions on the Production of Mycelial Biomass and Exopolysaccharide by Submerged Culture of a Rooting Shank Medicinal Mushroom, Oudemansiella radicata (Relhan) Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Jinn-Chyi Wang, Chiu-Yeh Wu, Yeu-Pyng Lin
Optimization of Submerged Fermentation Conditions for Lovastatin Production by the Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 5
Zeki Yildiz, Mustafa Yamac, Burcu Atli
Isolation and Characterization of Lectin from the Artist's Conk Medicinal Mushroom, Ganoderma applanatum (Agaricomycetes), and Evaluation of Its Antiproliferative Activity in HT-29 Colon Cancer Cells
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Ashok Kumar Pandurangan, Norhaizan Mohd. Esa, Sekar Kumaran, Ramalingam Shenbhagaraman