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Critical Reviews™ in Neurobiology

年間 3 号発行

ISSN 印刷: 0892-0915

ISSN オンライン: 2375-0014

SJR: 0.121

The Mouse Cerebellar Cortex in Organotypic Slice Cultures: An In Vitro Model to Analyze the Consequences of Mutations and Pathologies on Neuronal Survival, Development, and Function

巻 18, 発行 1-2, 2006, pp. 179-186
DOI: 10.1615/CritRevNeurobiol.v18.i1-2.180
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要約

Thin acute slices and dissociated cell cultures taken from different parts of the brain have been widely used to examine the function of the nervous system, neuron-specific interactions, and neuronal development (specifically, neurobiology, neuropharmacology, and neurotoxicology studies). Here, we focus on an alternative in vitro model: brain-slice cultures in roller tubes, initially introduced by Beat Gähwiler for studies with rats, that we have recently adapted for studies of mouse cerebellum. Cultured cerebellar slices afford many of the advantages of dissociated cultures of neurons and thin acute slices. Organotypic slice cultures were established from newborn or 10-15-day-old mice. After 3-4 weeks in culture, the slices flattened to form a cell monolayer. The main types of cerebellar neurons could be identified with immunostaining techniques, while their electrophysiological properties could be easily characterized with the patch-clamp recording technique. When slices were taken from newborn mice and cultured for 3 weeks, aspects of the cerebellar development were displayed. A functional neuronal network was established despite the absence of mossy and climbing fibers, which are the two excitatory afferent projections to the cerebellum. When slices were made from 10-15-day-old mice, which are at a developmental stage when cerebellum organization is almost established, the structure and neuronal pathways were intact after 3-4 weeks in culture. These unique characteristics make organotypic slice cultures of mouse cerebellar cortex a valuable model for analyzing the consequences of gene mutations that profoundly alter neuronal function and compromise postnatal survival.

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  1. Tzur-Gilat Aya, Ziv Yael, Mittelman Leonid, Barzilai Ari, Shiloh Yosef, Studying the cerebellar DNA damage response in the tissue culture dish, Mechanisms of Ageing and Development, 134, 10, 2013. Crossref

  2. Bakota Lidia, Brandt Roland, , 276, 2009. Crossref

  3. Pham Anh H., Chan David C., Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures, in Mitochondrial Function, 547, 2014. Crossref

  4. del Río José Antonio, Soriano Eduardo, Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration, Nature Protocols, 5, 2, 2010. Crossref

  5. Schubert Manja, Panja Debabrata, Haugen Mette, Bramham Clive R., Vedeler Christian A., Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis, Acta Neuropathologica, 128, 6, 2014. Crossref

  6. Lossi Laura, Alasia Silvia, Salio Chiara, Merighi Adalberto, Cell death and proliferation in acute slices and organotypic cultures of mammalian CNS, Progress in Neurobiology, 88, 4, 2009. Crossref

  7. Canterini Sonia, Bosco Adriana, Carletti Valentina, Fuso Andrea, Curci Armando, Mangia Franco, Fiorenza Maria Teresa, Subcellular TSC22D4 Localization in Cerebellum Granule Neurons of the Mouse Depends on Development and Differentiation, The Cerebellum, 11, 1, 2012. Crossref

  8. Hoff Mariana Leivas Müller, Fabrizius Andrej, Czech-Damal Nicole U., Folkow Lars P., Burmester Thorsten, Lionetti Vincenzo, Transcriptome Analysis Identifies Key Metabolic Changes in the Hooded Seal (Cystophora cristata) Brain in Response to Hypoxia and Reoxygenation, PLOS ONE, 12, 1, 2017. Crossref

  9. Tal Efrat, Shiloh Yosef, Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures, in ATM Kinase, 1599, 2017. Crossref

  10. de Almeida-Leite Camila Megale, Arantes Rosa Maria Esteves, Primary culture of glial cells from mouse sympathetic cervical ganglion: A valuable tool for studying glial cell biology, Journal of Neuroscience Methods, 194, 1, 2010. Crossref

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