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International Journal on Algae

年間 4 号発行

ISSN 印刷: 1521-9429

ISSN オンライン: 1940-4328

SJR: 0.168 SNIP: 0.377 CiteScore™:: 0.6 H-Index: 11

Indexed in

Rapid Method for Simultaneous Discrimination of Microalgae and Determination on Biochemical Composition based on Vibrational Spectroscopy

巻 19, 発行 4, 2017, pp. 385-396
DOI: 10.1615/InterJAlgae.v19.i4.80
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要約

Fourier-transform infrared (FT-IR) spectroscopy is a high-resolution spectroscopic method used in whole-organism molecular fingerprinting. This analysis helps for the characteristic determination of macromolecular spectrum in intact cells. Spectral analysis can discriminate, classify, and identify the microorganisms as composition of macromolecules is different between strains of the same species. In the present investigation, four different microalgae were studied for their variations in spectral data, which showed striking differences on their major macromolecules. Scenedesmus obliquus (Turp.) Kütz. have intense peaks in the lipids and protein spectral regions when compared to the other species. Chlorella vulgaris Beij., Ch. pyrenedosa Chick and Euglena gracilis Klebs possessed moderate and less intense peaks in sub sequential order in all the five spectral regions. Using FT-IR spectra, hierarchical clustering analyses resulted in a dendrogram with clear discrimination between species according to their phenotype variations. To support this, phylogenetic analysis using 16S rDNA microbial barcode sequences of these microalgal strains was evaluated and the resulting phylogram showed the same cluster pattern as revealed by FT-IR based dendrogram. Hence, this study concludes that the combination of phylogenetic analysis and the FTIR spectra provides an effective way of distinguishing the species as well as the advantage of simultaneous determination of biochemical composition of the species.

によって引用された
  1. Sureshkumar Pandian, Thomas Jibu, Strategic growth of limnic green microalgae with phycoremediation potential for enhanced production of biomass and biomolecules for sustainable environment , Environmental Science and Pollution Research, 26, 34, 2019. Crossref

  2. Sureshkumar Pandian, Thomas Jibu, Exploring the distinctiveness of biomass and biomolecules from limnic microalgae of unexplored waters of Noyyal River, Western Ghats, for exploitation, Environmental Science and Pollution Research, 27, 18, 2020. Crossref

  3. Thomas Jibu, Joseph Daliya, Sureshkumar Pandian, Stress induced production of high-value organic compounds from freshwater microalgae, Algologia, 30, 3, 2020. Crossref

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