RT Journal Article
ID 070449f81d3a8124
A1 Kała, Katarzyna
A1 Krakowska, Agata
A1 Gdula-Argasinska, Joanna
A1 Opoka, Wlodzimierz
A1 Muszynska, Bozena
T1 Assessing the Bioavailability of Zinc and Indole Compounds from Mycelial Cultures of the Bay Mushroom Imleria badia (Agaricomycetes) Using In Vitro Models
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2019
FD 2019-04-11
VO 21
IS 4
SP 343
OP 352
K1 Imleria badia
K1 mycelium from in vitro culture
K1 extraction into artificial digestive system
K1 indole compounds,
zinc
K1 CaCo-2 cells
K1 medicinal mushrooms
AB Zinc and indole compounds demonstrate anti-inflammatory, antidepressant, and antioxidant activity. Edible mushrooms are good sources of these substances. Therefore, in this study, we aimed to study the accumulation,
release, and absorption of zinc and indole compounds from mycelial cultures of Imleria badia species using
in vitro models. Samples were analyzed using the atomic absorption spectroscopy method and the reversed-phase high-performance liquid chromatography method. The highest quantities of zinc were detected in the material grown on zinc hydrogen aspartate-enriched media (176.01 mg/100 g dry weight [d.w.]). In addition, the quantity of zinc in the control biomass was approximately 12.13 mg/100 g d.w. After passive transport, the amount of zinc was detected to be around 1.40 mg/100 g d.w., whereas after active transport with CaCo-2 cells, the quantity of zinc ranged from 0.46 mg/100 g d.w. to 12.72 mg/100 g d.w. Among the organic compounds, four indole compounds were qualitatively
identified, including 5-hydroxy-l-tryptophan, melatonin, l-tryptophan, and 5-methyltryptamine. These results indicate that mushrooms and their in vitro cultures not only synthesize and accumulate these compounds, but also potentially release them into the gastrointestinal tract where they can be absorbed by the human body, which is reflected as a specific health benefit.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2b9ba90674ebcc50,070449f81d3a8124.html