RT Journal Article
ID 120acc867ef59a7e
A1 Habijanic, Jozica
A1 Svagelj, Mirjan
A1 Berovic, Marin
A1 Boh, Bojana
A1 Wraber, Branka
T1 Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2009
FD 2009-11-16
VO 11
IS 4
SP 409
OP 418
K1 medicinal mushrooms
K1 Ganoderma lucidum
K1 Grifola frondosa
K1 submerged and solid-state cultivation
K1 immunostimulatory effects
K1 human peripheral blood mononuclear cells
K1 mushroom polysaccharides
K1 cytokines
AB Original strains of Ganoderma lucidum (MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid-state cultivation. In G. lucidum after 14 days of submerged fed-batch cultivation, up to 17.0 g L−1 dry fungal biomass was produced. Extracellular (1.7 g L−1) and intracellular (0.45 g L−1) polysaccharide fractions were isolated, while in 18 days of solid-state cultivation, 5.77 mg/g of extracellular and 1.45 mg/g of intracellular polysaccharide were produced at the end of the cultivation period. In 28 days of submerged cultivation of G. frondosa, 3.65 mg L−1 of extracellular and 1.30 mg L−1 of intracellular polysaccharide were produced. In 38 days of solid-state cultivation, 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, gel, and affinity chromatography. The isolated polysaccharides were mainly β-D-glucans. Immunostimulatory effects of isolates were tested for induction of cytokine (TNF-α and IFN-γ) synthesis in human peripheral blood mononuclear cells (PBMC) isolated from buffy coat.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4554be083929b23c,120acc867ef59a7e.html