RT Journal Article ID 120acc867ef59a7e A1 Habijanic, Jozica A1 Svagelj, Mirjan A1 Berovic, Marin A1 Boh, Bojana A1 Wraber, Branka T1 Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae) JF International Journal of Medicinal Mushrooms JO IJM YR 2009 FD 2009-11-16 VO 11 IS 4 SP 409 OP 418 K1 medicinal mushrooms K1 Ganoderma lucidum K1 Grifola frondosa K1 submerged and solid-state cultivation K1 immunostimulatory effects K1 human peripheral blood mononuclear cells K1 mushroom polysaccharides K1 cytokines AB Original strains of Ganoderma lucidum (MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid-state cultivation. In G. lucidum after 14 days of submerged fed-batch cultivation, up to 17.0 g L−1 dry fungal biomass was produced. Extracellular (1.7 g L−1) and intracellular (0.45 g L−1) polysaccharide fractions were isolated, while in 18 days of solid-state cultivation, 5.77 mg/g of extracellular and 1.45 mg/g of intracellular polysaccharide were produced at the end of the cultivation period. In 28 days of submerged cultivation of G. frondosa, 3.65 mg L−1 of extracellular and 1.30 mg L−1 of intracellular polysaccharide were produced. In 38 days of solid-state cultivation, 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, gel, and affinity chromatography. The isolated polysaccharides were mainly β-D-glucans. Immunostimulatory effects of isolates were tested for induction of cytokine (TNF-α and IFN-γ) synthesis in human peripheral blood mononuclear cells (PBMC) isolated from buffy coat. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4554be083929b23c,120acc867ef59a7e.html