RT Journal Article
ID 266f20a049accda5
A1 Fujimiya, Yoshiaki
A1 Yamamoto, Hajimu
A1 Noji, Masahide
A1 Suzuki, Ikukatsu
T1 Peroral Effect on Tumor Progression of Soluble &beta;-(1, 6)-Glucans Prepared by Acid Treatment from <i>Agaricus blazei</i> Murr. (Agaricaceae, Higher Basidiomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2000
FD 2000-03-01
VO 2
IS 1
OP 7
AB Orally deliverable anticancer agents are becoming increasingly important in cost reduction of regimens for disorders that require protracted treatment and for patients' increasing preference and improved quality of life. The present study describes a simple procedure for the development of orally formulated anticancer agents of natural origin. The tumoricidal effects of natural products and purified extracts have previously been examined in single-grafted Meth-A tumor-bearing BALB/c mice by intratumoral injection of extracts from <i>Agaricus blazei</i>. Intratumoral administration of an ammonium oxalate-soluble/water- and ethanol-insoluble fraction 3, resulted in marked tumor regression, but continuous oral administration of feed containing fraction 3 from day 4 or 18 prior to tumor inoculation to day 21 after inoculation did not result in tumor regression. Intratumoral administration of fractions prepared by acid hydrolysis of either fraction 3 (ATF) or whole organisms (wATF) resulted in tumor regression almost identical to that seen using fraction 3. Oral administration of either soluble ATF or wATF also resulted in significant suppression of the progressive tumor, wATF being more active than ATF. ATF and wATF are composed predominantly of &beta;-(1, 6)-glucans with apparent molecular masses of 10 kDa, with some unknown metabolite contamination (<20%). These results demonstrate that simple acid treatment of whole natural basidiomycetous products consisting mainly of &beta;-(1, 6)-glucans can offer a valid approach to the development of anticancer agents that are <i>orally</i> effective, thus eliminating the need for multiple purification steps.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,684673684ea939cb,266f20a049accda5.html