RT Journal Article ID 2b819a517a17a56d A1 Feng, Jie A1 Feng, Na A1 Yang, Yan A1 Liu, Fang A1 Zhang, Jingsong A1 Jia, Wei A1 Lin, Chi-Chung T1 Simple and Reproducible Two-Stage Agitation Speed Control Strategy for Enhanced Triterpene Production by Lingzhi or Reishi Medicinal Mushrooms, Ganoderma lucidum ACCC G0119 (Higher Basidiomycetes) Based on Submerged Liquid Fermentation JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2016-01-22 VO 17 IS 12 SP 1151 OP 1159 K1 medicinal mushrooms K1 Ganoderma lucidum K1 batch fermentation K1 triterpenes K1 two-stage control strategy, agitation speed AB Triterpenes are important anticancer agents produced by batch submerged liquid fermentation, with the medicinal mushroom Ganoderma lucidum ACCC G0119, which was investigated under various dissolved oxygen levels by varying agitation speeds. Three kinetic parameters were analyzed: specific mycelial growth rate (μsmg), specific glucose consumption rate (qsgc), and specific triterpene production rate (qstp). High concentration, yield, and productivity of triterpenes were achieved by developing a simple and reproducible two-stage agitation speed control strategy. At the first 40 h, agitation speed was controlled at 150 rpm to obtain the quickest peak qstp for triterpene production, subsequently agitation speed was controlled at 100 rpm to maintain high qstp for high triterpene accumulation. The maximum concentration of triterpenes reached 0.086 g/l with the yield of 6.072 g/kg and the productivity of 6.532 × 10−4 g/(l·h), which were 39.61%, 36.48%, and 49.22%, respectively, better than the best results controlled by fixed agitation speeds. Conceivably, such a triterpene fermentation production strategy would be useful for industrial large-scale production of triterpenes with G. lucidum. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2d1fd1fc0b9784bf,2b819a517a17a56d.html