RT Journal Article
ID 2b819a517a17a56d
A1 Feng, Jie
A1 Feng, Na
A1 Yang, Yan
A1 Liu, Fang
A1 Zhang, Jingsong
A1 Jia, Wei
A1 Lin, Chi-Chung
T1 Simple and Reproducible Two-Stage Agitation Speed Control Strategy for Enhanced Triterpene Production by Lingzhi or Reishi Medicinal Mushrooms, Ganoderma lucidum ACCC G0119 (Higher Basidiomycetes) Based on Submerged Liquid Fermentation
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2015
FD 2016-01-22
VO 17
IS 12
SP 1151
OP 1159
K1 medicinal mushrooms
K1 Ganoderma lucidum
K1 batch fermentation
K1 triterpenes
K1 two-stage control strategy,
agitation speed
AB Triterpenes are important anticancer agents produced by batch submerged liquid fermentation, with
the medicinal mushroom Ganoderma lucidum ACCC G0119, which was investigated under various dissolved oxygen
levels by varying agitation speeds. Three kinetic parameters were analyzed: specific mycelial growth rate (μsmg), specific glucose consumption rate (qsgc), and specific triterpene production rate (qstp). High concentration, yield, and productivity of triterpenes were achieved by developing a simple and reproducible two-stage agitation speed control strategy. At the first 40 h, agitation speed was controlled at 150 rpm to obtain the quickest peak qstp for triterpene production, subsequently agitation speed was controlled at 100 rpm to maintain high qstp for high triterpene accumulation. The maximum concentration of triterpenes reached 0.086 g/l with the yield of 6.072 g/kg and the productivity of 6.532 × 10−4 g/(l·h), which were 39.61%, 36.48%, and 49.22%, respectively, better than the best results controlled by fixed agitation speeds. Conceivably, such a triterpene fermentation production strategy would be useful for industrial
large-scale production of triterpenes with G. lucidum.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2d1fd1fc0b9784bf,2b819a517a17a56d.html