RT Journal Article
ID 6c17828e0fce47b8
A1 Delben, Juliana Aparecida 
A1 Murata, Ramiro Mendonca 
A1 Wei, Xi 
A1 Castro, Myrella Lessio 
A1 Assuncao, Wirley Goncalves 
A1 da Silva, Nelson Renato Franca Alves 
A1 Duarte, Simone 
T1 Low-Temperature Plasma: An Effective Approach Against <i>Candida albicans</i> Biofilm
JF Plasma Medicine
JO PMED
YR 2014
FD 2015-02-11
VO 4
IS 1-4
SP 231
OP 244
K1 low-temperature plasma
K1 oral biofilm
K1 <i>Candida albicans</i>
AB This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour <i>Candida albicans</i> biofilm. A growth inhibition zone test was conducted with agar plates inoculated with <i>C. albicans</i> and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); &minus;application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The <i>C. albicans</i> biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on <i>C. albicans</i> biofilm.
PB Begell House
LK https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,700f28e67d84b510,6c17828e0fce47b8.html