Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
Journal of Environmental Pathology, Toxicology and Oncology
Fator do impacto: 1.241 FI de cinco anos: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i1.110
11 pages

Low Doses of Heavy Metals Disrupt Normal Structure and Function of Rat Platelets

Siragam Vinaya Kumar
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Rakhi Bose
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Shelley Bhattacharya
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India

RESUMO

Aggregation, an important property of platelets, plays a key role in the coagulation of blood and is potentiated by ADP and adrenaline, whereas cAMP acts as a strong inhibitor. Because of the rise in the heavy metal load in the environment, more studies are necessary to look at its subtle effects in the animal system, wherein platelet structure and function can be targeted. We carried out structural analyses under phase contrast and scanning electronmicroscopy of the platelets prepared from 1/10 LD50 metal-treated rats. After mercury (Hg) and arsenic (As) treatment, the cell margins appeared irregular and wavy, with small pseudopodia-like protrusions from the surface. Cadmium (Cd) treatment caused loss of the general spindle shape, and the platelets assumed a round spongy appearance. All metal treatments effected enhanced collagen-induced aggregation and inhibited ADP and arachidonic acid-induced aggregation, whereas epinephrine accelerated aggregation in Hg and Cd treatment but inhibited aggregation in As treatment. Cd proved a potent inhibitor of phosphodiesterase, increasing the cAMP level in in vitro treatments at equimolar (5, 10, and 20 pmoles) concentrations. The rate of aggregation was enhanced with all the agonists used in in vitro Hg and As treatments, with concomitant reduction in cAMP, while Cd inhibited platelet aggregation. Thus, we can conclude that cytosolic cAMP, which is decreased by the metal-induced inhibition of phosphodiesterase, is a regulator molecule in platelet aggregation. Furthermore, it is also abundantly clear that equimolar doses of metals are not always equitoxic. Therefore, the action of each xenobiotic is not only due to the concentration used but also to the type of xenobiotic depending on its unique mechanism of action.


Articles with similar content:

Rising of Constitutive NO-Synthase Coupling and Hemoglobin Content in Erythrocytes of Old Animals by Iron- Containing Herbal Drug
International Journal of Physiology and Pathophysiology, Vol.8, 2017, issue 3
S. I. Uretii, Anatoliy V. Kotsuruba, Bogdan S. Kopyak
Cold Physical Plasma Treatment Alters Redox Balance in Human Immune Cells
Plasma Medicine, Vol.3, 2013, issue 4
Sander Bekeschus, Axel Kramer, Kai Masur, Klaus-Dieter Weltmann, Thomas von Woedtke
Nitric Oxide's Contribution to Selective Apoptosis Induction in Malignant Cells through Multiple Reaction Steps
Critical Reviews™ in Oncogenesis, Vol.21, 2016, issue 5-6
Georg Bauer
Protective Role of Curcumin and Flunixin Against Acetic Acid−Induced Inflammatory Bowel Disease via Modulating Inflammatory Mediators and Cytokine Profile in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 4
G BOOBALAN, Matukumalli Usha Rani, C.S.V. Satish Kumar, Alla Gopala Reddy, Rasakatla Dileep, Matham Vijay Kumar
Radioprotection by Copper and Zinc Complexes of 5-Aminosalicylic Acid: A Preliminary Study
Journal of Environmental Pathology, Toxicology and Oncology, Vol.27, 2008, issue 2
M. K. Unnikrishnan, S. K. Mantena, K. Chandrasekharan