Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
Journal of Environmental Pathology, Toxicology and Oncology
Fator do impacto: 1.625 FI de cinco anos: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i1.110
11 pages

Low Doses of Heavy Metals Disrupt Normal Structure and Function of Rat Platelets

Siragam Vinaya Kumar
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Rakhi Bose
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Shelley Bhattacharya
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India

RESUMO

Aggregation, an important property of platelets, plays a key role in the coagulation of blood and is potentiated by ADP and adrenaline, whereas cAMP acts as a strong inhibitor. Because of the rise in the heavy metal load in the environment, more studies are necessary to look at its subtle effects in the animal system, wherein platelet structure and function can be targeted. We carried out structural analyses under phase contrast and scanning electronmicroscopy of the platelets prepared from 1/10 LD50 metal-treated rats. After mercury (Hg) and arsenic (As) treatment, the cell margins appeared irregular and wavy, with small pseudopodia-like protrusions from the surface. Cadmium (Cd) treatment caused loss of the general spindle shape, and the platelets assumed a round spongy appearance. All metal treatments effected enhanced collagen-induced aggregation and inhibited ADP and arachidonic acid-induced aggregation, whereas epinephrine accelerated aggregation in Hg and Cd treatment but inhibited aggregation in As treatment. Cd proved a potent inhibitor of phosphodiesterase, increasing the cAMP level in in vitro treatments at equimolar (5, 10, and 20 pmoles) concentrations. The rate of aggregation was enhanced with all the agonists used in in vitro Hg and As treatments, with concomitant reduction in cAMP, while Cd inhibited platelet aggregation. Thus, we can conclude that cytosolic cAMP, which is decreased by the metal-induced inhibition of phosphodiesterase, is a regulator molecule in platelet aggregation. Furthermore, it is also abundantly clear that equimolar doses of metals are not always equitoxic. Therefore, the action of each xenobiotic is not only due to the concentration used but also to the type of xenobiotic depending on its unique mechanism of action.


Articles with similar content:

The Influence of Habitat Conditions on the Properties of Eggs Proteins of Silver Carp
Hydrobiological Journal, Vol.36, 2000, issue 2
Yu. D. Konovalov
Effect of Corvitin on Secretory Processes and Blood Flow in the Rat Gastric Mucosa
International Journal of Physiology and Pathophysiology, Vol.4, 2013, issue 4
Stanislav P. Veselsky, Tetyana V. Vovkun, Vasyl A. Baranowskyy, Lydia Y. Shtanova, Petro I. Yanchuk
Effects of several common chemical fertilizers on Tolypothrix scytonemoides (Gardner) Geitler
International Journal on Algae, Vol.8, 2006, issue 1
Anand Narayanaswamy, Elango Kathirvel, Uma Maheshwari Rajendran
Calcium-Sensing Receptor Arbitrates Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells via the G Protein-PLC-IP3 Pathway
Critical Reviews™ in Eukaryotic Gene Expression, Vol.29, 2019, issue 1
Zhengying Duan, Ting Gao, Zuowu Jiao, Wei Liu, Yongxiang Tian, Keli Yang, Hui Zhang, Khalid Mehmood, Zhenyu Chang, Fangyan Yuan, Zewen Liu, Muhammad Taslim Ghori, Xiaoying Pei, Rui Guo, Muhammad Ijaz
Reversal of Carbon Tetrachloride-Induced Hepatic Injury by Aqueous Extract of Artemisia absinthium in Sprague-Dawley Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.31, 2012, issue 4
Monika Sharma, Sangeeta Shukla