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Journal of Environmental Pathology, Toxicology and Oncology
Fator do impacto: 1.241 FI de cinco anos: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v21.i3.50
6 pages

Cigarette Smoking Induces Apoptosis in Rat Testis

C. B. Dhabuwala
Departments of Urology, Wayne State University, School of Medicine, Detroit, Michigan
Joseph C. Dunbar
Departments of Physiology, Wayne State University, School of Medicine, Detroit, Michigan
Haikun Li
Department of Urology, Wayne State University, School of Medicine, Suite 1017,4160 John R Street, Detroit MI 48201
Atul Rajpurkar
Departments of Urology, Wayne State University, School of Medicine, Detroit, Michigan
Yang Jiang
Departments of Urology, Wayne State University, School of Medicine, Detroit, Michigan

RESUMO

In a previous study we demonstrated the deleterious effect of cigarette smoke on spermatogenesis in the testis of peripubertal Sprague-Dawley rats. In this study we investigated the development of apoptosis as a possible contributing factor to the pathogenic mechanism underlying these effects. Peripubertal rats were exposed to cigarette smoke with the Walton Horizontal Smoking Machine. Similarly, age-matched control rats were exposed to room air with the smoking machine. Rats from both groups were sacrificed after 45 days of treatment and the testes were removed. Testes were stained utilizing the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining technique. DNA fragmentation was further evaluated using gel electrophoresis. There was a significant increase in the incidence ofapoptosis in the treated group compared to the control group as demonstrated by the larger amount of tubules containing і3 apoptotic bodies in the smoke-exposed group, that is, 36% versus 14% in the control group (p < 0.05). Agarose gel electrophoresis demonstrated the DNA ladder in the treated group but not in the control animals. In conclusion, chronic cigarette smoke induces apoptosis in the rat testis. Apoptosis may be one of the pathogenic mechanisms responsible for defective spermatogenesis in the rat following chronic cigarette smoking.