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Journal of Environmental Pathology, Toxicology and Oncology
Fator do impacto: 1.241 FI de cinco anos: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v31.i2.10
pages 87-94

Effects of β-Lapachone on the Production of Th1 and Th2 Cytokines in C57BL/6 Mice

Myungwon Choi
Department of Microbiology, Kosin University College of Medicine, Busan, Korea
Indal Park
Department of Microbiology, Kosin University College of Medicine, Busan, Korea
Jaehyun Ju
Department of Food Science and Nutrition, Pusan National University, Busan, Korea
Kun-Young Park
Department of Food Science and Biotechnology, College of Life Sciences, Cha University, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea
Kwang Hyuk Kim
Department of Microbiology, Kosin University College of Medicine, Busan, Korea

RESUMO

In this study, we investigated the effects of β-lapachone (β-lap) on the production of cytokines in C57BL/6 mice. The culture supernatants of splenocytes exposed to β-lap plus lipopolysaccharide or concanavalin A (Con A) were harvested to determine Th1 (tumor necrosis factor-α, interferon-γ, interleukin [IL]-1β, IL-2, IL-17A, and IL-12) and Th2 (IL-4, IL-5, IL-6, and IL-10) cytokines. IL-2, a Th1 cytokine, was expressed at higher levels in splenocytes treated with β-lap and with lipopolysaccharide plus β-lap. Expression of Th2 cytokines, including IL-4, IL-5, IL-6, and IL-10, was lower in the culture supernatants of mouse splenocytes exposed to Con A plus β-lap than in supernatants from cells that were exposed to Con A alone. These findings demonstrate that β-lap downregulates the immediate hypersensitivity reaction induced by Con A. Therefore, Th2 responses involve the downregulation of IL-4, IL-5, IL-6, and IL-10, preventing the occurrence of allergies and various manifestations of allergic inflammation. These results suggest that β-lap may have potential preventive or adjunct anti-inflammatory therapy applications because of its function in modulating the production of cytokines. However, further in vivo investigations of this process are necessary to elucidate the mechanisms involved.


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