Publicou 4 edições por ano
ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
Indexed in
Comparison of Genotoxicity of Textile Dyestuffs in Salmonella Mutagenicity Assay, In Vitro Micronucleus Assay, and Single Cell Gel/Comet Assay
RESUMO
The mutagenicity of textile dyes is an important consideration for the assurance of consumer protection and work safety. The mutagenicity testing of textile dyestuffs is crucial for accurately predicting health risks for consumers and workers exposed to dyes. Unfortunately, these data are often lacking. We studied the genotoxic activity of ten selected commercial textile dyestuffs, which are made up of mixtures of azo dyes and azo metal complex dyes as well as two anthraquinone dyestuffs. We used the Salmonella mutagenicity assay and cultured human keratinocytes (HaCaT cell line). In the S. typhimurium strain TA98, with and without S9, eight often dyestuffs investigated, and in strain TA 100, with and without S9, six often dyes caused frameshift mutations and base-pair substitutions in the dose range of 15000 μg/plate in a dose-related manner. All dyes, including those negative in the Salmonella mutagenicity assay, induced clastogenic effects in the in vitro micronucleus (MN) test in HaCaT cells as direct-acting mutagens in the concentration range of 5150 μg/mL and with maximum MN frequencies between 1.1 and 7.2%, compared to negative controls that showed 0.20.4% MN cells. In the single cell gel/comet assay, all ten dyestuffs investigated caused DNA damage in HaCaT keratinocytes. The alkaline (pH >13) version used is capable of detecting DNA single strand breaks, alkali-labile sites, and DNADNA/DNAprotein cross-linking. Under the conditions of these screening tests, the textile dyes investigated are direct-acting genotoxic substances. The HaCaT cells testing protocol proposed has been shown to be an appropriate test system for evaluating mutagenicity of textile dyes on a base level.
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Dönbak L., Rencüzoğullari E., Topaktas M., Sahin G., A biomonitoring study on the workers from textile dyeing plants, Russian Journal of Genetics, 42, 6, 2006. Crossref
-
Tsuboy M.S., Angeli J.P.F., Mantovani M.S., Knasmüller S., Umbuzeiro G.A., Ribeiro L.R., Genotoxic, mutagenic and cytotoxic effects of the commercial dye CI Disperse Blue 291 in the human hepatic cell line HepG2, Toxicology in Vitro, 21, 8, 2007. Crossref
-
Manerikar R. S., Apte A. A., Ghole V. S., In vitrogenotoxicity of fly ash leachate in earthworm coelomocytes, Toxicological & Environmental Chemistry, 90, 2, 2008. Crossref
-
Klemola Kaisa, Pearson John, Liesivuori Jyrki, Lindström-Seppä Pirjo, Evaluating the toxicity of fabric extracts using the hepa-1 cytotoxicity test, the HaCaT cytotoxicity test and the spermatozoa motility inhibition test, Journal of the Textile Institute, 100, 4, 2009. Crossref
-
Chequer Farah Maria Drumond, Angeli José Pedro Friedmann, Ferraz Elisa Raquel Anastácio, Tsuboy Marcela Stefanini, Marcarini Juliana Cristina, Mantovani Mário Sérgio, de Oliveira Danielle Palma, The azo dyes Disperse Red 1 and Disperse Orange 1 increase the micronuclei frequencies in human lymphocytes and in HepG2 cells, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 676, 1-2, 2009. Crossref
-
Préfontaine David, Morin André, Jumarie Catherine, Porter Andrew, In vitro bioactivity of combustion products from 12 tobacco constituents, Food and Chemical Toxicology, 44, 5, 2006. Crossref
-
Bafana Amit, Jain Minakshi, Agrawal Gaurav, Chakrabarti Tapan, Bacterial reduction in genotoxicity of Direct Red 28 dye, Chemosphere, 74, 10, 2009. Crossref
-
Zeller Andreas, Pfuhler Stefan, N-acetylation of three aromatic amine hair dye precursor molecules eliminates their genotoxic potential, Mutagenesis, 29, 1, 2014. Crossref
-
Marathe Sunil D., Shrivastava Vinod S., Removal of textile dye Reactive Blue 59 by using Nb2O5as a photocatalyst, Desalination and Water Treatment, 2014. Crossref
-
Jung Daun, Che Jeong-Hwan, Lim Kyung-Min, Chun Young-Jin, Heo Yong, Seok Seung Hyeok, Discrimination of skin sensitizers from non-sensitizers by interleukin-1α and interleukin-6 production on cultured human keratinocytes, Journal of Applied Toxicology, 36, 9, 2016. Crossref
-
Tafurt-Cardona Yaliana, Suares-Rocha Paula, Fernandes Thaís Cristina Casimiro, Marin-Morales Maria Aparecida, Cytotoxic and genotoxic effects of two hair dyes used in the formulation of black color, Food and Chemical Toxicology, 86, 2015. Crossref
-
Mariselvam R., Ranjitsingh A. J. A., Mosae Selvakumar P., Alarfaj Abdullah A., Munusamy Murugan A., Spectral Studies of UV and Solar Photocatalytic Degradation of AZO Dye and Textile Dye Effluents Using Green Synthesized Silver Nanoparticles, Bioinorganic Chemistry and Applications, 2016, 2016. Crossref
-
Tang Alan Y.L., Lo Chris K.Y., Kan Chi-wai, Textile dyes and human health: a systematic and citation network analysis review, Coloration Technology, 134, 4, 2018. Crossref
-
Barathi Selvaraj, Karthik Chinnannan, S Nadanasabapathi, Padikasan Indra Arulselvi, Biodegradation of textile dye Reactive Blue 160 by Bacillus firmus (Bacillaceae: Bacillales) and non-target toxicity screening of their degraded products, Toxicology Reports, 7, 2020. Crossref
-
Dutta Joydeep, Chitosan-Based Composite Beads for Removal of Anionic Dyes, in Textile Wastewater Treatment, 2022. Crossref