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Journal of Environmental Pathology, Toxicology and Oncology

Publicou 4 edições por ano

ISSN Imprimir: 0731-8898

ISSN On-line: 2162-6537

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 2.4 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.8 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.5 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00049 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.59 SJR: 0.429 SNIP: 0.507 CiteScore™:: 3.9 H-Index: 49

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Culture of Human Fibrosarcoma HT-1080 Cells in Presence of Fibronectin Activates MMP-2

Volume 25, Edição 4, 2006, pp. 667-678
DOI: 10.1615/JEnvironPatholToxicolOncol.v25.i4.60
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RESUMO

The importance of tumor cell surface integrin receptors in regulation of matrix metalloproteinase (MMP) expression and function has been reported. Integrin-ECM ligand interaction leads to phosphorylation of focal adhesion kinase (FAK) and activation of mitogen activated protein kinase (MAPK) pathways. In this present study, we cultured human fibrosarcoma cells, HT-1080, in presence of fibronectin to study fibronectin-integrin mediated modulation of MMP activity. Methods: HT-1080 cells were cultured in serum free medium (SFCM) in presence of fibronectin, SFCM was collected, and gelatin zymography was performed. Western blot and immunocytochemistry were performed with HT-1080 cells cultured in presence of fibronectin. Results: Culture of HT-1080 cells in presence of 50 μg/1.5 ml fibronectin led to expression of pro-MMP-9 and activation of MMP-2 within 1 hr. When HT-1080 cells were treated with PI-3K inhibitor (LY294002) and grown in presence of fibronectin, MMP-2 activation was partially inhibited, but when cells were treated with ERK inhibitor (PD98059) and grown in presence of fibronectin, MMP-2 activation was almost completely inhibited. Tyrosine phosphorylation of FAK and ERK were increased in HT-1080 cells grown in presence of fibronectin. Processing of MT1-MMP was also observed in HT-1080 cells grown in presence of fibronectin. The reorganization of actin filaments in fibronectin treated HT-1080 cells was also noticeable. Conclusions: Our findings indicate that culture of HT-1080 cells in SFCM in presence of fibronectin perhaps generates a signaling cascade that leads to expression of pro-MMP-9 and activation of MMP-2 within 1 hr. The signaling pathway activated seems to be the FAK/ERK pathway.

CITADO POR
  1. Maity Gargi, Fahreen Shabana, Banerji Aniruddha, Roy Choudhury Paromita, Sen Triparna, Dutta Anindita, Chatterjee Amitava, Fibronectin–integrin mediated signaling in human cervical cancer cells (SiHa), Molecular and Cellular Biochemistry, 336, 1-2, 2010. Crossref

  2. Dutta Anindita, Sen Triparna, Banerji Aniruddha, Das Shamik, Chatterjee Amitava, Studies on Multifunctional Effect of All-Trans Retinoic Acid (ATRA) on Matrix Metalloproteinase-2 (MMP-2) and Its Regulatory Molecules in Human Breast Cancer Cells (MCF-7), Journal of Oncology, 2009, 2009. Crossref

  3. Zuber Chantal, Knackmuss Stefan, Zemora Georgeta, Reusch Uwe, Vlasova Ekaterina, Diehl Daniela, Mick Vera, Hoffmann Karin, Nikles Daphne, Fröhlich Thomas, Arnold Georg J., Brenig Bertram, Wolf Eckhard, Lahm Harald, Little Melvyn, Weiss Stefan, Invasion of Tumorigenic HT1080 Cells Is Impeded by Blocking or Downregulating the 37-kDa/67-kDa Laminin Receptor, Journal of Molecular Biology, 378, 3, 2008. Crossref

  4. Sen Triparna, Moulik Shuvojit, Dutta Anindita, Choudhury Paromita Roy, Banerji Aniruddha, Das Shamik, Roy Madhumita, Chatterjee Amitava, Multifunctional effect of epigallocatechin-3-gallate (EGCG) in downregulation of gelatinase-A (MMP-2) in human breast cancer cell line MCF-7, Life Sciences, 84, 7-8, 2009. Crossref

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