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Journal of Environmental Pathology, Toxicology and Oncology
Fator do impacto: 1.625 FI de cinco anos: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i4.50
pages 323-331

Photoprotective Effect of Calluna vulgaris Extract Against UVB-Induced Phototoxicity in Human Immortalized Keratinocytes

Maria Perde-Schrepler
The Oncology Institute Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania
Gabriela Chereches
Ion Chiricuta Oncology Institute, Cluj-Napoca, Romania
Ioana Carmen Brie
The Oncology Institute Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania
Piroska Virag
Ion Chiricuta Oncology Institute, Cluj-Napoca, Romania
Otilia Barbos
Ion Chiricuta Oncology Institute, Cluj-Napoca, Romania
Olga Soritau
Ion Chiricuta Oncology Institute, Cluj-Napoca, Romania
Corina Bianca Tatomir
The Oncology Institute Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania
Eva Fischer-Fodor
The Oncology Institute Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania; Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania
Gabriela Adriana Filip
Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Clinicilor Street, No. 1-2, 400006, Cluj-Napoca, Romania
Laurian Vlase
Departments of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania
Ion Dan Postescu
Department of Radiobiology and Tumor Biology, Prof. I. Chiricuta Oncologic Institute, Cluj-Napoca, Romania

RESUMO

There is an increasing interest in the use of natural antioxidants as photoprotective agents against skin damages produced by ultraviolet radiation. The aim of our study was to investigate the protective effect of a Calluna vulgaris extract in human keratinocytes (HaCaT) exposed to ultraviolet B (UVB) radiation. HaCaT cells were treated with C. vulgaris extract 30 minutes prior to irradiation with UVB. The protective effect was evaluated by assessing cell viability using tetrasolium salt (MTT) assay; the generation of lipid peroxides was evaluated using malondialdehide assay (MDA); and DNA damage was evaluated using the comet assay and the quantification by ELISA of specific DNA photolesions [i.e., cyclobutane-pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs)]. After irradiation with cytotoxic doses of UVB (300 and 500 mJ/cm2), HaCaT cells pretreated with C. vulgaris extract (50 μg GAE/ml) showed significantly increased viability compared to control cells exposed to UVB only. Irradiation alone increased MDA levels in a dose-dependent fashion. Pretreatment with 12 μg GAE/ml extract lowered MDA levels both at 100 mJ/cm2 (ρ<0.01) and 300 mJ/cm2 (ρ<0.001). Treatment with C. vulgaris extract before exposure to UVB also reduced DNA damage: Lesion scores in a comet assay were significantly reduced at UVB doses of 50 mJ/cm2 (ρ<0.01) and 100 mJ/cm2 (ρ<0.05), while CPDs and 6-4PPs (via ELISA) were significantly lower after irradiation with 100 mJ/cm2 in the protected cells (ρ<0.05 for CPDs and ρ<0.001 for 6-4PPs). These results recommend the use of the C. vulgaris extract as photoprotective agent, in combination with sunscreens and/or other natural products with similar or complementary properties.


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