Publicou 4 edições por ano
ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
Indexed in
Gene Expression Profiling of Di-n-Butyl Phthalate in Normal Human Mammary Epithelial Cells
RESUMO
Studies show that female workers in the personal-care industry have an increased risk of developing cancer believed to be the result of increased exposure to toxic and/or carcinogenic chemicals found in cosmetics, hair dyes, and nail polish. One chemical found in multiple personal-care products, di-n-butyl phthalate (DBP), is a known endocrine disruptor and has been found in increased levels in women of childbearing age. The goal of this study was to elucidate mechanisms of phthalate toxicity in normal human cells to provide information concerning interindividual variation and gene-environment interactions. Normal human mammary epithelial cell strains were obtained from discarded tissues following reduction mammoplasty [Cooperative Human Tissue Network (sponsors: NCI/NDRI)]. Gene transcription in each cell strain was analyzed using high-density oligonucleotide DNA microarrays (U133A, Affymetrix™) and changes in the expression of selected genes were verified by real-time polymerase chain reaction (PCR) (ABI). DNA microarrays were hybridized with total RNA that was collected after DBP treatment for 5 hr and 10 hr. RNA was harvested from the vehicle control (acetone) at 10 hr. Data Mining Tool software (Affymetrix) was used to separate genes in clusters based on their expression patterns over time. Only 57 genes were found to be altered in all four cell strains following exposure to DBP. These included genes involved in fertility (inhibin, placental growth factor), immune response (tumor necrosis factor induced protein), and antioxidant status (glutathione peroxidase). Data from this study will help clarify the role of DBP in reproductive toxicity, and yield biomarkers of exposure for future epidemiology studies.
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Gwinn Maureen R., Weston Ainsley, Application of Oligonucleotide Microarray Technology to Toxic Occupational Exposures, Journal of Toxicology and Environmental Health, Part A, 71, 5, 2008. Crossref
-
Di-n-butyl phthalate [MAK Value Documentation, 2013], in The MAK-Collection for Occupational Health and Safety, 2013. Crossref
-
Koniecki Diane, Wang Rong, Moody Richard P., Zhu Jiping, Phthalates in cosmetic and personal care products: Concentrations and possible dermal exposure, Environmental Research, 111, 3, 2011. Crossref
-
Sengupta Krishanu, Kolla Jayaprakash Narayana, Bagchi Debasis, Bagchi Manashi, Applications of toxicogenomics in reproductive and developmental toxicology, in Reproductive and Developmental Toxicology, 2011. Crossref
-
Kang Ju-Chan, Jee Jung-Hoon, Koo Ja-Geun, Keum Yoo-Hwa, Jo Soo-Gun, Park Kwan Ha, Anti-oxidative status and hepatic enzymes following acute administration of diethyl phthalate in olive flounder Paralichthys olivaceus, a marine culture fish, Ecotoxicology and Environmental Safety, 73, 6, 2010. Crossref
-
Di-n-butylphthalat [MAK Value Documentation in German language, 2010], in The MAK-Collection for Occupational Health and Safety, 2012. Crossref
-
Gray Janet M., Rasanayagam Sharima, Engel Connie, Rizzo Jeanne, State of the evidence 2017: an update on the connection between breast cancer and the environment, Environmental Health, 16, 1, 2017. Crossref
-
Zhang Guang Ming, Lang Lang, Estrogenicity of Six Typical Aqueous Pollutants, Advanced Materials Research, 499, 2012. Crossref
-
Li Zengqiang, Yu Yige, Li Yang, Ma Feifei, Fang Yinghui, Ni Chaobo, Wu Keyang, Pan Peipei, Ge Ren-Shan, Taxifolin attenuates the developmental testicular toxicity induced by di-n-butyl phthalate in fetal male rats, Food and Chemical Toxicology, 142, 2020. Crossref
-
de Freitas Thiago, Zapaterini Joyce R., Moreira Cristiane M., de Aquino Ariana M., Alonso-Costa Luiz G., Bidinotto Lucas T., Kass Laura, Flaws Jodi A., Scarano Wellerson R., Barbisan Luis F., Prenatal exposure to a mixture of different phthalates increases the risk of mammary carcinogenesis in F1 female offspring, Food and Chemical Toxicology, 156, 2021. Crossref
-
He Lin, Michailidou Freideriki, Gahlon Hailey L., Zeng Weibin, Hair Dye Ingredients and Potential Health Risks from Exposure to Hair Dyeing, Chemical Research in Toxicology, 35, 6, 2022. Crossref