Publicou 4 edições por ano
ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
IF:
2.4
5-Year IF:
2.8
Immediacy Index:
0.5
Eigenfactor:
0.00049
JCI:
0.59
SJR:
0.429
SNIP:
0.507
CiteScore™::
3.9
H-Index:
49
Indexed in
Enhancement of Radiation Cytotoxicity in Murine Cancer Cells by Electroporation: In Vitro and In Vivo Studies
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RESUMO
Increasing evidence has accumulated in recent years to suggest that the cell membrane forms the vital common target for action by ionizing radiation and electroporation. The present work describes the use of electric pulses for enhancement of radiation-induced cytotoxicity of cancer cells both in vitro and in vivo. In vitro: low dose rate (0.37 Gy/min) Co60 γ-rays (2 Gy) in combination with electric pulses of square wave (2 kV/cm, 200 μs duration, 8 pulses/burst, 10 times) significantly enhanced the cytotoxicity in Ehrlich ascites carcinoma cells (EAC), probably through enhanced production of intracellular reactive oxygen species (ROS).The intracellular generation of ROS and changes in oxidative damage-mediated membrane fluidity were determined by fluorescence using DCH-FDA and DPH, respectively, as probes. Both radiation and electroporation, separately, have been observed to produce ROS in a dose-dependent fashion. We show that the combined treatment of cells with radiation and electroporation significantly increased intracellular ROS and changed membrane fluidity of EAC cells as compared to the effects by each individual treatment. In vivo studies have been carried out with murine fibrosarcoma as a model system. The localized treatment of a fibrosarcoma tumor, grown in the right hind leg of Swiss mice, had been carried out using radiation (Co60 γ-rays, 2 Gy, dose rate: 0.37 Gy /min) and electric pulses (1 kV/cm, 200 μs, 8 pulses/burst, 10 times). Studies on tumor growth kinetics have shown a significant growth delay (by 50% to that of control) 7 days after treatment of tumor with radiation and electroporation. The results suggest that radiocytotoxicity of tumor cells in vitro as well as in vivo were enhanced significantly by electric pulses, which may offer a potentially improved treatment of cancer.
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Kostoff Ronald N., Lau Clifford G.Y., Combined biological and health effects of electromagnetic fields and other agents in the published literature, Technological Forecasting and Social Change, 80, 7, 2013. Crossref
-
Shil Pratip, Bidaye Salil, Vidyasagar Pandit B, Analysing the effects of surface distribution of pores in cell electroporation for a cell membrane containing cholesterol, Journal of Physics D: Applied Physics, 41, 5, 2008. Crossref
-
Markelc B, Tevz G, Cemazar M, Kranjc S, Lavrencak J, Zegura B, Teissie J, Sersa G, Muscle gene electrotransfer is increased by the antioxidant tempol in mice, Gene Therapy, 19, 3, 2012. Crossref
-
Gupta Subash C., Hevia David, Patchva Sridevi, Park Byoungduck, Koh Wonil, Aggarwal Bharat B., Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy, Antioxidants & Redox Signaling, 16, 11, 2012. Crossref
-
Kranjc Simona, Kamensek Urska, Cemazar Maja, Sersa Gregor, Combined Treatment of Electrochemotherapy with Irradiation, in Handbook of Electroporation, 2016. Crossref
-
Mishra K, Membrane Electroporation and Emerging Biomedical Applications, in Handbook of Membrane Separations, 2008. Crossref
-
Teissie Justin, Involvement of Reactive Oxygen Species in Membrane Electropermeabilization, in Handbook of Electroporation, 2017. Crossref
-
Teissie Justin, Involvement of Reactive Oxygen Species in Membrane Electropermeabilization, in Handbook of Electroporation, 2017. Crossref
-
Kranjc Simona, Kamensek Urska, Čemažar Maja, Serša Gregor, Combined Treatment of Electrochemotherapy with Irradiation, in Handbook of Electroporation, 2017. Crossref
-
Kostoff Ronald N., Lau Clifford G. Y., Modified Health Effects of Non-ionizing Electromagnetic Radiation Combined with Other Agents Reported in the Biomedical Literature, in Microwave Effects on DNA and Proteins, 2017. Crossref
-
Golomb Beatrice Alexandra, Diplomats' Mystery Illness and Pulsed Radiofrequency/Microwave Radiation, Neural Computation, 30, 11, 2018. Crossref
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Nguyen Thanh‐Van, Wittayarat Manita, Do Lanh Thi Kim, Nguyen Thanh Van, Nii Masahiro, Namula Zhao, Kunihara Toshiki, Tanihara Fuminori, Hirata Maki, Otoi Takeshige, Effects of chlorogenic acid ( CGA ) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization , Animal Science Journal, 89, 8, 2018. Crossref
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Rezaee Zohre, Yadollahpour Ali, Bayati Vahid, Single Intense Microsecond Electric Pulse Induces Radiosensitization to Ionizing Radiation: Effects of Time Intervals Between Electric Pulse and Ionizing Irradiation, Frontiers in Oncology, 8, 2018. Crossref