Effect of UHMWPE Particle Size, Dose, and Endotoxin on in vitro Macrophage Response

Carie  Alley; Warren O.  Haggard; Richard A. Smith

doi:10.1615/JLongTermEffMedImplants.2014010273

Journal of Long-Term Effects of Medical Implants

Editor chefe: Subrata Saha (open in a new tab)

Publicou 4 edições por ano

ISSN Imprimir: 1050-6934

ISSN On-line: 1940-4379

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Effect of UHMWPE Particle Size, Dose, and Endotoxin on in vitro Macrophage Response

Volume 24, Edição 1, 2014, pp. 45-56

DOI: 10.1615/JLongTermEffMedImplants.2014010273

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Carie Alley
Smith & Nephew, Inc., 1450 E Brooks Rd, Memphis, TN 38116

Warren O. Haggard
Chair Excellence Associate Dean, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152

Richard A. Smith
Department of Orthopedic Surgery & Biomedical Engineering, University of Tennessee Campbell Clinic, Memphis, TN 38163

RESUMO

Ultra-high molecular weight polyethylene wear debris generated by a prosthetic hip or knee has been linked to osteolysis and the limited lifespan of the implant. However, research results are conflicting with regard to which characteristics of the polyethylene wear debris are most inflammatory. The goal of this study was to determine whether particle size, number, and the presence of endotoxin significantly contribute to increased secretion of pro-inflammatory mediators by macrophages in vitro in response to polyethylene wear debris generated by a hip simulator. The results show that the prevailing inflammatory factor is endotoxin. The macrophages released only minimal levels of TNF-α and IL-6 in response to cleaned polyethylene particles, but these cytokines were released in significantly higher amounts in response to particles spiked with lipopolysaccharide (LPS). The number (up to 500 particles per cell) and size of the particles tested in this study had no significant influence on any of the measured outputs (macrophage viability, TNF-α, IL-6, or PGE₂) unless associated with LPS.

Palavras-chave: cytokines, polyethylene, macrophage

CITADO POR

Greenfield Edward M., Do Genetic Susceptibility, Toll-like Receptors, and Pathogen-associated Molecular Patterns Modulate the Effects of Wear?, Clinical Orthopaedics and Related Research®, 472, 12, 2014. Crossref
Liu Yumei, Shi Feng, Gong Kemeng, Liu Yang, Zhi Wei, Weng Jie, Qu Shuxin, Study on critical-sized ultra-high molecular weight polyethylene wear particles loaded with alendronate sodium: in vitro release and cell response, Journal of Materials Science: Materials in Medicine, 28, 4, 2017. Crossref
Li Juehong, Li Yamin, Peng Xiaochun, Li Bin, Qin Hui, Chen Yunsu, In vivo analysis of the effects of CoCrMo and Ti particles on inflammatory responses and osteolysis, RSC Advances, 8, 10, 2018. Crossref
Vallés G., Vilaboa N., Osteolysis After Total Hip Arthroplasty: Basic Science, in Acetabular Revision Surgery in Major Bone Defects, 2019. Crossref
Lei Pengfei, Dai Zixun, Zhang Yu Shrike, Liu Hua, Niu Wanting, Li Kun, Wang Long, Hu Yihe, Xie Jie, Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis, Journal of Orthopaedic Surgery and Research, 14, 1, 2019. Crossref
Wang Shiwen, Feng Qiang, Sun Jiashu, Gao Feng, Fan Wei, Zhang Zhong, Li Xiaohong, Jiang Xingyu, Nanocrystalline Cellulose Improves the Biocompatibility and Reduces the Wear Debris of Ultrahigh Molecular Weight Polyethylene via Weak Binding, ACS Nano, 10, 1, 2016. Crossref

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