Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
Journal of Porous Media
Fator do impacto: 1.49 FI de cinco anos: 1.159 SJR: 0.504 SNIP: 0.671 CiteScore™: 1.58

ISSN Imprimir: 1091-028X
ISSN On-line: 1934-0508

Volumes:
Volume 22, 2019 Volume 21, 2018 Volume 20, 2017 Volume 19, 2016 Volume 18, 2015 Volume 17, 2014 Volume 16, 2013 Volume 15, 2012 Volume 14, 2011 Volume 13, 2010 Volume 12, 2009 Volume 11, 2008 Volume 10, 2007 Volume 9, 2006 Volume 8, 2005 Volume 7, 2004 Volume 6, 2003 Volume 5, 2002 Volume 4, 2001 Volume 3, 2000 Volume 2, 1999 Volume 1, 1998

Journal of Porous Media

DOI: 10.1615/JPorMedia.v12.i3.20
pages 213-220

Protein Crystal-Mediated Biotemplating

Noa Cohen-Hadar
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Yariv Wine
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Shira Lagziel-Simis
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Hila Moscovich-Dagan
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Yael Dror
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Felix Frolow
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Amihay Freeman
Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

RESUMO

Protein crystals, routinely prepared for the elucidation of protein 3-D structure by X-ray crystallography, present a highly accurate 3-D array of protein molecules. Along with the formation of this array, a complementary 3-D array of voids is also formed, with pattern, geometry, and size depending on the protein “building-block” and its packaging within the crystal. Here we present recent results demonstrating the feasibility of using the pores of protein crystals as a biotemplate for the preparation of composite materials. Our previously developed methods of monitoring the preservation of the 3-D protein crystal template throughout biotemplating of chemically cross-linked hydrogels and the elucidation of the mechanism of protein crystal cross-linking by glutaraldehyde were applied in this study to enable and demonstrate the feasibility of protein crystal-mediated biotemplating resulting in a 3-D array of silver particles located within the pores of the crystal. Our accumulated results indicate that the pool of protein crystals may be successfully exploited as a source of biotemplates for the fabrication of novel composite materials, in contrast to the common consideration of protein crystals as labile structures, useful only for the elucidation of the 3-D structure of proteins.


Articles with similar content:

LASER PROCESSING OF SEMICONDUCTOR NANOMATERIALS
Annual Review of Heat Transfer, Vol.20, 2017, issue 1
Costas Grigoropoulos
TEMPERATURE SENSITIVE PARTICLES FOR THE 3-D SIMULTANEOUS MEASUREMENT OF VELOCITY AND TEMPERATURE
Turbulence and Shear Flow Phenomena -1 First International Symposium, Vol.0, 1999, issue
Hitoshi Sugiyama, Nao Ninomiya, Mitsunobu Akiyama
TEMPERATURE SENSITIVE PARTICLES FOR THE 3-D SIMULTANEOUS MEASUREMENT OF VELOCITY AND TEMPERATURE
TSFP DIGITAL LIBRARY ONLINE, Vol.1, 1999, issue
Hitoshi Sugiyama, Nao Ninomiya, Mitsunobu Akiyama
HEAT AND FLUID FLOW ANALYSIS DURING CASTING PROCESS USING AN IMPLICIT FILLING ALGORITHM
International Heat Transfer Conference 11, Vol.20, 1998, issue
Kwan-Soo Lee, Ik-Tae Im, Woo-Seung Kim
ESR-Cell Based on Disk Dielectric Resonator with Tunable Marker
Telecommunications and Radio Engineering, Vol.68, 2009, issue 10
Т. V. Kalmykova, S. I. Tarapov, A. A. Girich