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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimir: 1947-5764
ISSN On-line: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2017019487
pages 159-173

Viability and Cell Biology for HeLa and Vero Cells after Exposure to Low-Temperature Air Dielectric Barrier Discharge Plasma

Ioana Cristina Gerber
Alexandru Ioan Cuza University of Iasi, Faculty of Physics, Iasi Plasma Advanced Research Center (IPARC), Iasi, 700506, Romania
Cosmin Teodor Mihai
Alexandru Ioan Cuza University of Iasi, Interdisciplinary Research Department, Field Science, Iasi, 700506, Romania
Lucian Gorgan
Alexandru Ioan Cuza University of Iasi, Faculty of Biology, Iasi, 700506, Romania
Mitica Ciorpac
Alexandru Ioan Cuza University of Iasi, Interdisciplinary Research Department, Field Science, Iasi, 700506, Romania
Alexandru Nita
Alexandru Ioan Cuza University of Iasi, Faculty of Biology, Iasi, 700506, Romania
Valentin Pohoata
Alexandru Ioan Cuza University of Iasi, Faculty of Physics, Iasi Plasma Advanced Research Center (IPARC), Iasi, 700506, Romania
Ilarion Mihaila
Alexandru Ioan Cuza University of Iasi, Integrated Center of Environmental Science Studies in the North-Eastern Development Region (CERNESIM), Iasi, 700506, Romania
Ionut Topala
Alexandru Ioan Cuza University of Iasi, Faculty of Physics, Iasi Plasma Advanced Research Center (IPARC), Iasi, 700506, Romania

RESUMO

Cancer study is among the hottest topics in plasma medicine. Differential killing of cancer cells and controllable selectivity may be achieved using exposure to plasma generated by various atmospheric pressure discharges. In this article, we discuss the possibility of generating low-temperature air plasma directly inside standard 24- and 96-well cell-culture plates and its subsequent use to achieve selective cytotoxicity. After exposure, cell viability was assessed and molecular biology tests carried out to understand the effect of plasma on cell biology. HeLa (neoplastic cells) and Vero (normal cells) cultures were exposed to air dielectric barrier discharge plasma. We performed viability assessment using the 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide assay at 8 and 24 hr after treatment and found a reduction in cell viability, with a variable amplitude of effect. The cytotoxicity of plasma on HeLa cells at 8 hr was 59.92% and reached 68.58% by 24 hr. The cytotoxic effect on Vero cells was limited to 37.75% at 8 hr and only 6.33% at 24 hr. General cell biology analyses were assessed using flow cytometry and gene expression tracking.