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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimir: 1947-5764
ISSN On-line: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2016016783
pages 107-113

Use of Green Fluorescent Protein for Rapid Assessment of the Bactericidal Activity under Cold Plasma Irradiation

Zhigang Ke
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
Lamei Li
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
Jingwen Yan
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
Shaopeng Chen
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China
Vandana Miller
C&J Nyheim Plasma Institute, Drexel University, Camden, New Jersey 08103
Alexander A. Fridman
C&J Nyheim Plasma Institute, Drexel University, Camden, New Jersey 08103
Qing Huang
Anhui Jianzhu University, Hefei, China; Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China

RESUMO

Bacterial inactivation by nonthermal or cold plasma is affected by many factors, yet the mechanisms have not been thoroughly clarified. Developing effective methods for rapid evaluation of bactericidal activity of cold plasma is thus crucial for not only optimizing the experimental conditions for the best sterilization effect, but also revealing the underlying mechanisms. Although conventional methods such as determination of colony-forming units are still the standard method for assessment of bacterial inactivation efficiency, they are time-consuming and cannot provide real-time measurement. In this work, green fluorescent protein (GFP) was utilized as the indicator for monitoring the bactericidal activity of cold plasma irradiation. GFP-expressing recombinant Escherichia coli was exposed to discharge plasma, and the intracellular fluorescence signal was detected with a flow cytometer. We found that the bacterial GFP fluorescence intensity decreased exponentially with plasma exposure time, and the result was in good accordance with the inactivation curve obtained by measuring colony-forming units. As such, this work demonstrates that GFP is useful for high-throughput screening assay for antimicrobial activity of nonthermal plasma irradiation.


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