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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Imprimir: 1947-5764
ISSN On-line: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2016015848
pages 13-19

Characterization of Reactive Oxygen/ Nitrogen Species Produced in PBS and DMEM by Air DBD Plasma Treatments

Ilaria Trizio
Department of Chemistry, University of Bari "Aldo Moro", Bari, Italy
Eloisa Sardella
CNR, IMIP, Italy
Vito Rizzi
Department of Chemistry, University of Bari "Aldo Moro", Bari, Italy
Giorgio Dilecce
NANOTEC Institute, CNR, Bari, Italy
Pinalysa Cosma
Department of Chemistry, University of Bari "Aldo Moro", Bari, Italy
Michael Schmidt
Leibniz Institute for Plasma Science and Technology e.V. (INP Greifswald), 17489 Greifswald, Germany
Thomas von Woedtke
Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
Roberto Gristina
NANOTEC Institute, CNR, Bari, Italy
Pietro Favia
Department of Chemistry, University of Bari "Aldo Moro", Bari, Italy; NANOTEC Institute, CNR, Bari, Italy

RESUMO

Atmospheric pressure air dielectric barrier discharges were applied to phosphate-buffered saline and complete Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum to investigate their oxidative chemical modifications induced in liquids that are relevant for cell culture experiments in the field of Plasma Medicine. The study mainly identified long-living reactive species. Hydrogen peroxide, superoxide anion radicals, and nitrite and nitrate ions were detected in DMEM. The density of the reactive species was correlated with the energy dose delivered to the liquids by the discharges.


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