Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimir: 1947-5764
ISSN On-line: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2018027349
pages 15-22

Comparison of Cellular Sensitivity to a Split Radiation Dose and a Combination of a Single Radiation Dose and Electromagnetic Field Exposure

Angela Chinhengo
Division of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa
Antonio Serafin
Division of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa
John Akudugu
Division of Radiobiology, Department of Medical Imaging and Clinical Oncology, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa


HIV-positive individuals, who are at high risk of developing cancers such as Kaposi's sarcoma, tend to be more sensitive to ionizing radiation and are at a higher risk of developing severe side effects during radiotherapy. To enhance therapeutic benefit in this patient cohort, new and noninvasive methods are needed to sensitize cancer cells and reduce therapeutic doses. To partially address this need, the effects of 100 and 1000 Hz electromagnetic fields (EMF) on the radiosensitivity of Chinese hamster lung fibroblasts (V79) and human melanoma cells (MeWo) were evaluated, using the colony forming assay. The surviving fraction (SF) of V79 cells exposed to a 1000 Hz field for 30 min, followed by 2 Gy of X-rays 6 h later (SF = 0.6833 ± 0.0067), was significantly higher than that obtained when cells were irradiated twice with 1.5 Gy X-rays, 6 h apart (SF = 0.5620 ± 0.0026; P = 0.0008). On the other hand, the combination of EMF exposure and irradiation was more toxic (SF = 0.3350 ± 0.0050) in the melanoma cells than the split radiation treatment (SF = 0.3825 ± 0.0035; P = 0.0008). These data suggest that use of EMF may significantly reduce the total radiation dose during radiotherapy and minimize normal tissue toxicity without compromising tumor control.


  1. Cattelan AM, Calabro ML, De Rossi A, Aversa SML, Barbierato M, Trevenzoli M, Gasperini P, Zanchetta M, Cadrobbi P, Monfardini S, Chieco-Bianchi L., Long-term clinical outcome of AIDS-related Kaposi's sarcoma during highly active antiretroviral therapy. Int J Oncol. 2005;27:779-85.

  2. Berson AM, Quivey JM, Harris JW, Wara WM. , Radiation therapy for AIDS related Kaposi's sarcoma. Int J Radiat Oncol Biol Phys. 1990;19:569-75.

  3. Watkins EB, Findlay P, Gelmann E, Lane HC, Zabell A. , Enhanced mucosal reactions in AIDS patients receiving oropharyngeal irradiation. Int J Radiat Oncol Biol Phys. 1987;13:1403-8.

  4. Stelzer JK, Griffin TW. , A randomized prospective trial of radiation therapy for AIDS-associated Kaposi's sarcoma. Int J Radiat Oncol Biol Phys. 1993;27:1057-61.

  5. Mourad WF, Hu KS, Ishihara D, Shourbaji RA, Lin W, Kumar M, Jacobson AS, Tran T, Manolidis S, Urken M, Persky M, Harrison L., Tolerance and toxicity of primary radiation therapy in the management of seropositive HIV patients with squamous cell carcinoma of the head and neck. Laryngoscope. 2013;123:1178-83.

  6. Herd O, Francies F, Slabbert J, Baeyens A. , The effect of HIV and antiretroviral therapy on chromosomal radiosensitivity. J AIDS Clin Res. 2014;5:397.

  7. Ulrike K, Markus H, Thomas H, Ellen H, Barbara S, Rainer F, Distel LV. , NNRTI-based antiretroviral therapy may increase risk of radiation induced side effects in HIV-l-infected patients. Radiother Oncol. 2015;116:323-30.

  8. Kirson ED, Dbaly V, Tovarys F, Vymazal J, Soustiel JF, Itzhaki A, Mordechovich D, Steinberg-Shapira S, Gurvich Z, Schneiderman R, Wasserman Y, Salzberg M, Ryffel B, Goldsher D, Dekel E, Palti Y., Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors. Proc Natl Acad Sci USA. 2007;104:10152-7.

  9. Barbault A, Costa FP, Bottger B, Munden RF, Bomholt F, Kuster N, Pasche B., Amplitude-modulated electromagnetic fields for the treatment of cancer: discovery of tumor-specific frequencies and assessment of a novel therapeutic approach. J Exp Clin Cancer Res. 2009;28:51.

  10. Verginadis I, Velalopoulou A, Karagounis I, Simos I, Peschos D, Karkabounas S, Evangelou A., Beneficial effects of electromagnetic radiation in cancer. In: Bashir SO, editor. Electromagnetic radiation. Shanghai: InTech; 2012. p. 249-68.

  11. Simko M, Kriehuber R, Weiss DG, Luben RA. , Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines. Bioelectromagnetics. 1998;19:85-91.

  12. Tofani S. , Electromagnetic energy as a bridge between atomic and cellular levels in the genetics approach to cancer treatment. Curr Top Med Chem. 2015;15:572-8.

  13. Tofani S, Barone D, Cintarino M, de Santi MM, Ferrara A, Orlassino R, Ossola P, Peroglio F, Rolfo K, Ronchetto F., Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics. 2001;22:419-28.

  14. Sarimov R, Markova E, Johansson F, Jenssen D, Belyaev I. , Exposure to ELF magnetic field tuned to Zn inhibits growth of cancer cells. Bioelectromagnetics. 2005;26:631-8.

  15. Crocetti S, Beyer C, Schade G, Egli M, Frohlich J, Franco-Obregon A. , Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability. PLoS One. 2013;8(9):e72944.

  16. Vadala M, Morales-Medina JC, Vallelunga A, Palmieri B, Laurino C, Iannitti T. , Mechanisms and therapeutic effectiveness of pulsed electromagnetic field therapy in oncology. Cancer Med. 2016;5:3128-39.

  17. Chinhengo A, Serafin A, Hamman B, Akudugu J. , Electromagnetic fields induce frequency-dependent radioprotection and radiosensitization in in vitro cell cultures. Plasma Med. 2018;8:163-75.

  18. Albino AP, Vidal MJ, McNutt NS, Shea CR, Prieto VG, Nanus DM, Palmer JM, Hayward NK. , Mutation and expression of the p53 gene in human malignant melanoma. Melanoma Res. 1994;4:35-45.

  19. Chaung W, Mi L-J, Boorstein RJ. , The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair. Nucleic Acids Res. 1997;25:992-4.

  20. Clarke AR, Purdie CA, Harrison DJ, Morris RG, Bird CC, Hooper ML, Wyllie AH. , Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature. 1993;362:849-52.

  21. Zhivotovsky B, Kroemer G. , Apoptosis and genomic instability. Nat Rev Mol Cell Biol. 2004;5:752-62.

  22. Bujko K, Suit HD, Springfield DS, Convery K. , Wound healing after preoperative radiation for sarcoma of soft tissue. Surg Gynecol Obstet. 1993;176:124-34.

  23. Gu Q, Wang D, Cui C, Gao Y, Xia G, Cui X. , Effects of radiation on wound healing. J Environ Pathol Toxicol Oncol. 1998;17:117-23.

  24. Kunisada T, Ngan SY, Powell G, Choong PFM. , Wound complications following pre-operative radiotherapy for soft tissue sarcoma. Eur J Surg Oncol. 2002;28:75-9.

  25. Mendelsohn FA, Divino CM, Reis ED, Kerstein MD. , Wound care after radiation therapy. Adv Skin Wound Care. 2002;15:216-24.

  26. Haubner F, Ohmann E, Pohl F, Strutz J, Gassner HG. , Wound healing after radiation therapy: review of the literature. Radiat Oncol. 2012;7:162.

  27. Griffin AM, Dickie CI, Catton CN, Chung PWM, Ferguson PC, Wunder JS, O'Sullivan B., The influence of time interval between preoperative radiation and surgical resection on the development of wound healing complications in extremity soft tissue sarcoma. Ann Surg Oncol. 2015;22:2824-30.

  28. Akudugu JM, Bell RS, Catton C, Davis AM, O'Sullivan B, Waldron J, Wunder JS, Hill RP., Clonogenic survival and cytokinesis-blocked binucleation of skin fibroblasts and normal tissue complications in soft tissue sarcoma patients treated with preoperative radiotherapy. Radiother Oncol. 2004;72:103-12.

Articles with similar content:

Electromagnetic Fields Induce Frequency-Dependent Radioprotection and Radiosensitization in In Vitro Cell Cultures
Plasma Medicine, Vol.8, 2018, issue 2
Antonio Serafin, Bianca Hamman, John Akudugu, Angela Chinhengo
Photoprotective Effect of Calluna vulgaris Extract Against UVB-Induced Phototoxicity in Human Immortalized Keratinocytes
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 4
Olga Soritau, Corina Bianca Tatomir, Gabriela Chereches, Ion Dan Postescu, Maria Perde-Schrepler, Ioana Carmen Brie, Laurian Vlase, Gabriela Adriana Filip, Otilia Barbos, Eva Fischer-Fodor, Piroska Virag
Antihypoxic Activities of the Golden Chanterelle Mushroom, Cantharellus cibarius (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 4
Masoumeh Khalili, Farnoosh Omrani, Mohammad Ali Ebrahimzadeh, Mohammad Karami
Safety Evaluation of an Aqueous Extract of Termitomyces robustus (Agaricomycetes) in Wistar Rats
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 2
Okezie Emmanuel, Victor Chibueze Ude, Ozioma Onyero, Eziuche Amadike Ugbogu, Emmanuel Iroha Akubugwo, Cynthia Ibe, Nduka Ozoji Okomba
Preclinical Evaluation of Concurrent Medicinal Mushroom-Based Immune-Enhancement Supplementation in Dogs Undergoing Chemotherapy for Various Cancers
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 2
Sharon Y. West, Marie N. Mullins, Matt P. Cleaver, Britt M. Gianotti