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Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimir: 1947-5764
ISSN On-line: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2018028140
pages 255-268

Indirect Inactivation of Candida guilliermondii by Using a Plasma Synthetic Jet Actuator: Effect of Advected Charged Particles

Gabriele Neretti
Electrical, Electronic and Information Engineering Department, University of Bologna, Italy
Anna C. Ricchiuto
Electrical, Electronic and Information Engineering Department, University of Bologna, Italy
Roberta Galuppi
Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy
Giovanni Poglayen
Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy
Benedetto Morandi
Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy
Ester Marotta
Department of Chemical Sciences, University of Padova, Italy
Cristina Paradisi
Department of Chemical Sciences, University of Padova, Italy
Francesco Tampieri
Department of Chemical Sciences, University of Padova, Italy
Carlo A. Borghi
Department of Electrical, Electronic and Information Engineering, University of Bologna, Viale del Risorgimento 2, 40136 Bologna, Italy

RESUMO

Plasma synthetic jet actuators (PSJAs) demonstrated their ability to produce a tubular flow normal to the surface where the dielectric barrier discharge (DBD) is ignited. These fluid dynamics actuators enhance the delivery of reactive and charged species toward the target to be treated. In this work, the efficacy of an octagonal PSJA used to indirectly treat Candida guilliermondii, both in agar substrate and in saline solution, was experimentally studied. The inactivation effect of free charges advected by the induced flow has been investigated as well by interposing a metallic mesh between the actuator surface and the treated sample. Therefore, the passage of charges and their migration toward the sample can either be allowed or prevented simply by having the mesh floating or connected to the ground, respectively. A 4 log and a 2 log colony-forming unit (CFU) reduction were observed for agar substrate and saline solution, respectively, at the highest energy dose treatment with the grid grounded. Under the same conditions but with the grid at floating potential, the presence of advected charges gives an additional 1 log CFU. In addition to biological tests, chemical analyses have been carried out to identify the most important modifications produced by the induced flow into treated water. Results presented in this work demonstrate the efficacy of PSJAs in indirect treatment of C. guilliermondii, both on agar substrate and saline solution, and the inactivation properties of free charges advected by this type of fluid dynamics actuator.