Inscrição na biblioteca: Guest
Plasma Medicine

Publicou 4 edições por ano

ISSN Imprimir: 1947-5764

ISSN On-line: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

Plasma-Patterned Polydimethylsiloxane Surface With Single-Step Coating with a Mixture of Vitronectin and Albumin Enables the Formation of Small Discs and Spheroids of Human Induced Pluripotent Stem Cells

Volume 4, Edição 1-4, 2014, pp. 165-176
DOI: 10.1615/PlasmaMed.2014012077
Get accessDownload

RESUMO

Plasma treatment is an easy method of cleaning and hydrophilizing glass and polymer surfaces to facilitate adsorption of cell-adhesive proteins. Thus a patterned plasma treatment is useful for fabricating cell adhesion patterns. We previously succeeded in producing human induced pluripotent stem cell (hiPSC) discs (2 mm in diameter) on plasma-patterned polydimethylsiloxane (PDMS) by single-step coating of a mixture of 2 ubiquitous proteins, vitronectin and γ-globulin. Under serum-free and feeder-free conditions, without any undefined cell adhesion molecules, γ-globulin blocked hiPSC adhesion on surfaces not treated with plasma. However, γ-globulin has low cost-effectiveness and availability, and the resulting disc diameter was too large for cell-based assays. We demonstrate that bovine serum albumin (BSA) can also be used to block hiPSC adhesion on plasma-untreated PDMS surfaces coated with vitronectin. We succeeded in creating small hiPSC discs (200 µm in diameter) using single-step coating of a mixture of vitronectin and BSA. The hiPSCs proliferated without escaping from the patterned area and finally spontaneously detached from the discs to form spheroids. We believe that our method for generating hiPSC discs and spheroids will be useful for developing new bioengineering devices to enhance cell differentiation and to test drug safety for human embryonic development, contributing to future medical applications.

CITADO POR
  1. Kondo Yuki, Hattori Koji, Tashiro Shota, Nakatani Eri, Yoshimitsu Ryosuke, Satoh Taku, Sugiura Shinji, Kanamori Toshiyuki, Ohnuma Kiyoshi, Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates, Journal of Bioscience and Bioengineering, 124, 2, 2017. Crossref

  2. Fernando W. T. Leon S., Kato Gen, Miyazaki Shota, Maruyama Kenshiro, Takahashi Kazumasa, Kikuchi Takashi, Ohnuma Kiyoshi, Sasaki Toru, Mask-Free Plasma Patterning for Biocompatible Material Using Atmospheric Pressure Plasma Jet, IEEE Transactions on Radiation and Plasma Medical Sciences, 4, 1, 2020. Crossref

  3. Fernando W. T. L. S., Takahashi Kazumasa, Kikuchi Takashi, Sasaki Toru, Effect of Additional Pin Electrode Placing at the Bottom of the Plasma Jet and Flow Behavior on Radical Generation and Propagation, IEEE Transactions on Plasma Science, 49, 1, 2021. Crossref

Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa Políticas de preços e assinaturas Begell House Contato Language English 中文 Русский Português German French Spain