Publicou 6 edições por ano
ISSN Imprimir: 1045-4403
ISSN On-line: 2162-6502
IF:
1.6
5-Year IF:
2.2
Immediacy Index:
0.3
Eigenfactor:
0.00058
JCI:
0.33
SJR:
0.345
SNIP:
0.46
CiteScore™::
2.5
H-Index:
67
Indexed in
Chromatin Structure and Nuclear Remodeling
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RESUMO
Nuclear architecture is remodeled during interphase in response to changes in gene activity as well as to changing structural and functional requirements during cell division. Using the monoclonal antibody mAb2A, we have identified two proteins that appear to play important roles in these processes: JIL-1 is a tandem serine-threonine kinase implicated in the regulation of chromatin structure, whereas Skeletor is a novel protein participating in structural nuclear remodeling during the cell cycle. Antibody staining and live imaging of JIL-1 -GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost twofold on the hypertranscribed male X chromosome compared with autosomes. We propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure. The other mAb2A antigen, Skeletor, is distributed in a nuclear meshwork pattern that can be observed in stereo pair images to reorganize during the cell cycle to form a spindle-like structure at prometaphase that is distinct from the microtubule spindle apparatus. Taking advantage of the powerful molecular and genetic approaches offered in Drosophila, the study of these two proteins promises to yield new insight into what defines nuclear architecture at the molecular level and how its remodeling is regulated.
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Sala Anna, Toto Maria, Pinello Luca, Gabriele Alessandra, Di Benedetto Valeria, Ingrassia Antonia M R, Lo Bosco Giosuè, Di Gesù Vito, Giancarlo Raffaele, Corona Davide F V, Genome-wide characterization of chromatin binding and nucleosome spacing activity of the nucleosome remodelling ATPase ISWI, The EMBO Journal, 30, 9, 2011. Crossref
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Walker Diana L., Wang Dong, Jin Ye, Rath Uttama, Wang Yanming, Johansen Jørgen, Johansen Kristen M., Skeletor, a Novel Chromosomal Protein That Redistributes during Mitosis Provides Evidence for the Formation of a Spindle Matrix, Journal of Cell Biology, 151, 7, 2000. Crossref
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Burgio Giosalba, Cipressa Francesca, Ingrassia Antonia Maria Rita, Cenci Giovanni, Corona Davide F. V., The histone deacetylase Rpd3 regulates the heterochromatin structure ofDrosophilatelomeres, Journal of Cell Science, 124, 12, 2011. Crossref
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Zhang Weiguo, Wang Yanming, Long Jin, Girton Jack, Johansen Jørgen, Johansen Kristen M., A Developmentally Regulated Splice Variant from the Complexlola Locus Encoding Multiple Different Zinc Finger Domain Proteins Interacts with the Chromosomal Kinase JIL-1, Journal of Biological Chemistry, 278, 13, 2003. Crossref
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Nickerson J., Experimental observations of a nuclear matrix, Journal of Cell Science, 114, 3, 2001. Crossref
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Zhang Weiguo, Jin Ye, Ji Yun, Girton Jack, Johansen Jørgen, Johansen Kristen M, Genetic and Phenotypic Analysis of Alleles of the Drosophila Chromosomal JIL-1 Kinase Reveals a Functional Requirement at Multiple Developmental Stages, Genetics, 165, 3, 2003. Crossref
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Li Yeran, Cai Weili, Wang Chao, Yao Changfu, Bao Xiaomin, Deng Huai, Girton Jack, Johansen Jørgen, Johansen Kristen M., Domain Requirements of the JIL-1 Tandem Kinase for Histone H3 Serine 10 Phosphorylation and Chromatin Remodeling in Vivo, Journal of Biological Chemistry, 288, 27, 2013. Crossref