RESUMO

The study was conducted on three groups of rats: Group I included Wistar rats with normal blood pressure (first control group); group II - rats with genetically determined hypertension (second control group); group III - rats with genetically determined hypertension under the influence of magnetic-laser power (experimental group). For the low-intensively magnetic-laser effect (MLE), we used a device MIT-MT, Ukraine, which was designed for the treatment by low-frequency magnetic field using optical flow of blue and red spectrum ranges. The MLI duration was 15 minutes for the blue range, and 25 minutes for the red one. Biochemical studies included the determination of the activity of isoenzymes of NO-synthase: constitutive (cNOS) and inducible (iNOS), the content of free hemoglobin, stable metabolites of NO, namely nitrite- (NO₂⁻) and nitrate- (NO₃⁻) anions, and resistance to erythrocyte acid hemolysis. In physiological study, the contractile activity of the aorta smooth muscles was measured. We have found that an exposure of rats with genetically determined hypertension to magnetic-laser action at red (630 nm wavelength) and blue (470 nm wavelength) optical range, even after a single session, leads to an increase in nitric oxide synthesis in the blood plasma. Our data indicate that a ten-day course of the magnetic-laser exposure to the optical flow of the blue spectral range turned out to be the most effective in the intensification of endogenous nitric oxide (an increase in NO₂⁻ and reduction of NO₃⁻) and endothelium-dependent responses of the aorta in rats with genetically determined hypertension. Moreover, after 10 sessions of magnetic-laser action using above spectrum, erythrocyte membrane gets stabilized.