Inscrição na biblioteca: Guest
International Journal of Physiology and Pathophysiology

Publicou 4 edições por ano

ISSN Imprimir: 2155-014X

ISSN On-line: 2155-0158

SJR: 0.116

Hydrogen Sulfide Inhibits Calcium-Induced Mitohondrial Permeability Transition Pore Opening in the Heart of Adult and Old Rats

Volume 3, Edição 2, 2012, pp. 135-147
DOI: 10.1615/IntJPhysPathophys.v3.i2.40
Get accessGet access

RESUMO

Effects of NaHS, a hydrogen sulfide donor, and L-cysteine, a substrate of its biosynthesis, on the sensitivity of the mitohondrial permeability transition pore (rnPTP) to its natural inductor Ca2+ were investigated on mitochondria isolated from the heart tissues of adult and old rats in experiments in vivo and in vitro. It has been shown that NaHS at concentrations in the range between 10−12 and 10−8 mol / l induced moderate mitochondrial swelling of the rat heart in calcium-free medium. At concentration of 10−10 mol / L, NaHS caused mitochondrial swelling, and changes in its maximum size (Δ) accounted for 11% and 15% in adult and old rats, respectively. An inhibitor of mitochondrial ATP-dependent potassium channels (KATP channels) 5-hydroxydecanoate (10−4 mol / l) reduced the mitochondrial swelling in the presence of NaHS (10−10 mol / l), which may indicate a contribution of these channels to calcium-independent membrane conductance in the heart organelles. Hydrogen sulfide donor in physio-logical concentrations of 10−6, 10−5 and 5·10−5mol / l depressed calcium-induced mPTP opening, indicating its protective effect on pore formation that comprised 31%, 76% and 77%, respectively in the heart of adult rats. However, in old animals this effect was observed only at NaHS concentrations of 10−5 mol / l. Thus, hydrogen sulfide donor within the studied concentrations resulted in ambiguous effects on mPTP opening: low concentrations (10−12 − 10−8 mol / l) increased mitochondrial swelling of the organelles, but its physiological concentrations (10−6 −5·10−5 mol / l) carried protective effect on calcium-induced mitochondrial swelling in rat hearts. Preincubation of isolated mitochondria with 5-hydroxydecanoate (10−4 mol / l) reduced the protective effect of NaHS (10−5 mol / l) relative to calcium-induced mPTP opening, indicating the possible involvement of mitochondrial KATP channels in hydrogen sulfide-dependent inhibition of pore formation in the heart. In experiments in vivo with a single intraperitoneal administration of either NaHS (10−4 mol / kg) or L-cysteine (10−3 mol / kg), a decrease in mPTP sensitivity to Ca2+ inductor in the heart of adult and old rats has been shown. L-cysteine turned out to be more effective to prevent calcium-induced mPTP opening compared with NaHS: a tenfold increase in Ca2+ concentration was observed, resulting in mitochondria swelling in the heart. In in vivo experiments, a specific inhibitor of cystathionine-γ-lyase propargylglycine (10−4 mol / kg) which is involved in the biosynthesis of hydrogen sulfide, induced a significant increase in mPTP sensitivity to the Ca2+ inductor due to two orders of magnitude decrease in its threshold concentration, which, in turn, causes swelling of the organelles. Therefore, endogenous hydrogen sulfide has been shown to be envolved in the regulation of pore formation in the heart. Thus, the data obtained indicate the participation of hydrogen sulfide in the modulation of the changes in the permeability of mitochondrial membranes, which may be important regulatory factor in the development of cardiovascular diseases.

CITADO POR
  1. Strutynska N.A., Korkach Yu.V., Mys L.A., Luchkova A.Yu., Sagach V.F., AMINOGUANIDINE RESTORES ENDOGENOUS HYDROGEN SULFIDE SYNTHESIS, CONSTITUTIVE NITRIC OXIDE SYNTHESIS, ENDOTHELIUM-DEPENDENT VASODILATION AND INHIBITS MITOCHONDRIAL PERMEABILITY TRANSITION PORE OPENING IN THE HEART OF OLD RATS, Fiziolohichnyĭ zhurnal, 65, 6, 2019. Crossref

  2. Strutynska N.A., Korkach Yu.P., Mys L.А., Luchkova А.Yu., Sagach V.F., L-CYSTEINE STIMULATES ENDOGENOUS HYDROGEN SULFIDE SYNTHESIS, SUPPRESSES OXIDATIVE STRESS AND MITOCHONDRIAL PERMEABILITY TRANSITION PORE OPENING IN THE HEART OF OLD RATS, Fiziolohichnyĭ zhurnal, 66, 2-3, 2020. Crossref

  3. Strutynska N.A., Goshovska Yu.V., Korkach Yu.P. , Mys L.A., Strutynskyi R.B. , Sagach V.F. , EFFECTS OF GLUTATHIONE ON THE EXPRESSION OF ATP-SENSITIVE POTASSIUM CHANNELS, MITOCHONDRIAL PORES, AND ON OXIDATIVE STRESS IN THE HEART OF OLD RATS, Fiziolohichnyĭ zhurnal, 66, 6, 2020. Crossref

815 Visualizações do artigo 7 downloads de artigos Métricas
815 VISUALIZAÇÕES 7 TRANSFERÊNCIAS 3 Crossref CITAÇÕES Google
Scholar
CITAÇÕES

Artigos com conteúdo semelhante:

Hydrogen Sulfide Inhibits Calcium-Induced Mitochondrial Permeability Transition Pore Opening in the Heart of Spontaneously Hypertensive Rats International Journal of Physiology and Pathophysiology, Vol.4, 2013, issue 4
Nataliya A. Strutynska, Galyna L. Vavilova, Vadim F. Sagach, Nataliya A. Dorofeyeva
Effect of NaHS, Hydrogen Sulfide Donor, on the Functional State of the Respiratory Chain in the Rat Heart Mitochondria International Journal of Physiology and Pathophysiology, Vol.5, 2014, issue 2
Nataliya A. Strutynska, Galyna L. Vavilova, Alina Yu. Budko, Vadim F. Sagach, Olena M. Semenykhina
Inhibition of Mitochondrial Hydrogen Sulfide Production Worsens Heart Contractility and Enhances Sensitivity of Mitochondrial Permeability Transition Pore to Calcium International Journal of Physiology and Pathophysiology, Vol.9, 2018, issue 2
Nataliya A. Strutynska, Alina Yu. Luchkova, Vadim F. Sagach, Yuliya V. Hoshovs'ka, Raisa A. Fedichkina
The Effect of Lomg-Term Exercise Training on the Changes in the Functional State of Heart duringIschemia-Reperfusion, the Sensitivity of Calcium- Induced Mitochondrial Permeability Transition Pore and the Uncoupling Protein 3 Expression International Journal of Physiology and Pathophysiology, Vol.1, 2010, issue 3
Snizhana V. Chorna, Nataliya A. Strutynska, Galyna L. Vavilova, Vadim F. Sagach, N. M. Gaidai, Sergiy O. Talanov
Activation of Endogenous Hydrogen Sulfide Synthesis Inhibits Mitochondrial Permeability Transition Pore Opening and Restores Constitutive NO-Synthase Coupling in Old Rat Heart International Journal of Physiology and Pathophysiology, Vol.9, 2018, issue 1
Nataliya A. Strutynska, V. R. Strutynskyi, Lidiya A. Mys, Vadim F. Sagach

Última edição

Systemic Toxic Dental Sealant-Induced Effect on Nervous Tissue in vitro O. A. Znachkova, Irina V. Lushnikova, Galina G. Skibo, M. Y. Antonenko, Nana V. Voitenko Impact of Various Polyphenols on Disturbance of Cholesterol Metabolism under Insulin Resistance V. P. Fylymonenko, A. L. Zagayko Target Correction of Mitochondrial Dysfunction in Parkinson's Disease by Capicor Iryna M. Mankovska, Kateryna V. Rosova, Olga O. Gonchar, Valentina I. Nosar, Larisa. V. Bratus, Tetyana I. Drevitska, Ivan D. Glazyrin, Nina V. Karasevich, Iryna M. Karaban Changes in Ongoing Activity and Electrophysiological Characteristics of the Rat Retinal Ganglion Cells in Diabetes Mellitus N. Ya. Martyniuk, V. Yu. Maslov, Helen E. Purnyn, Svetlana A. Fedulova, Nikolai S. Veselovsky Sex Characteristics of Hydrogen Sulfide Effect on Ischemia-Reperfusion in Rat Myocardium Andrii V. Melnyk, Nataliia V. Zaichko, Oleksii A. Khodakovskyi, Olga V. Khodakivska Cardiohemodynamic Changes in Focal Cerebral Ischemia Roman R. Sharipov, Nataliya A. Dorofeyeva, Vadim F. Sagach Cerebral Hemodynamics and Electroencephalographic Pattern in Patients with Symptomatic Epilepsy at Combined Treatment with Transcranial Direct Current Stimulation Kateryna V. Yatsenko Physiological Endurance of Skeletal Muscle Increases under C60 Fullerene Application before Physical Load Tetyana Yu. Matvienko, Danylo Zavodovskiy, Iryna V. Mishchenko, Kateryna I. Bogutska, Yuriy I. Prylutskyy, Sofia Paradizova, Larisa Bezuh, Uwe Ritter, Peter Scharff Possible Mechanisms Underlying Association between Thyroiditis and Thyroid Papillary Carcinoma Tamara M. Myshunina, Mykola D. Tronko Index, Volume 9, 2018
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa Políticas de preços e assinaturas Begell House Contato Language English 中文 Русский Português German French Spain