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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i4.10
pages 331-345

Study on the Analytical Method for Water-soluble Crude Polysaccharides in Culinary-Medicinal Mushrooms

Shu-Fang Lu
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Shu-Chi Tsai
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Hsiao-Chen Lin
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.
Weiguang Fu
Analysis Research and Service Center, Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.

RESUMO

Size exclusion high performance liquid chromatography (SE-HPLC) was used to analyze water extracts of edible medicinal mushrooms in this research. Fingerprint profiling of polysaccharides in edible medicinal mushrooms was utilized as the primary identification tool for qualitative analysis. Furthermore, this study successfully improves or corrects the overestimation problem in quantifying functional polysaccharide contents in phenol-sulfuric acid method. The comparison of 3 different pre-treatment methods including SE-HPLC fractionation, ultra-filtration (UF) fractionation and alcohol precipitation shows that SE-HPLC fractionation method not only can recover the loss of polysaccharides in UF or alcohol precipitation methods, but also is able to fully retain water-soluble crude polysaccharides with molecular weight > 10 kD, which are commonly regarded as functional components in medicinal mushrooms. Therefore, the combination of SE-HPLC fractionation and phenol-sulfuric acid quantification method is more accurate and suitable for determination of water-soluble crude polysaccharide in edible medicinal mushrooms.


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