Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.730
433 pages

Production of Ligninolytic Enzymes by Oyster Mushroom Pleurotus ostreatus (Jacq.:Fr.) P. Kumm. Under Different Nutritional Conditions

Nona A. Mikiashvili
Institute of Evolution, University of Haifa, Israel; Iv. Javakhishvili State University of Tbilisi, Department of Biochemistry and Biotechnology, Georgia; Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, USA

RESUMO

One of the effective methods for regulating and increasing the quantity of ligninolytic enzymes is the addition of the appropriate inducers to the nutrient medium. The ligninolytic enzymes of some white-rot fungi are synthesized only after the primary growth phase in response to nitrogen and carbon limitations in the medium. In the screening program, Pleurotus ostreatus appeared to be one of the best producers of extracellular laccase (Lac) and manganese peroxidase (MgP). Therefore, we compared the effect of different carbon and nitrogen sources, including lignocellulosic substrates, on P. ostreatus 98 oxidative enzyme production.
The secretion of Lac, MgP, and versatile peroxidase (VP) were significantly dependent on 11 types of carbon sources when species were grown in a medium with 2 g/L NH4NO3. Mandarin peels, which were used as one of the possible substrates, ensured very high yields of Lac (2375.5 U/L). Increasing its concentration in the nutrition medium from 20 g/L to 40 g/L resulted in a more than six-fold increase in Lac activity. Carboxylmethylcellulose, gluconic acid, glucose, and cellobiose (10 g/L) appeared to be poor growth substrates for Lac production. Moreover, results of this study showed that the activity of the enzyme was at its lowest level on medium containing avicel and mannitol.
Lignin degradation of P. ostreatus 98 on culture media with 10 g/L glucose and 11 types of nitrogen sources (0.5 g/L) was suppressed. This species showed high Lac activity in the presence of peptone, kazein acid, and corn step liquor (887.1 U/L, 802.3 U/L, and 674.3 U/L, respectively). In contrast, medium with NaNO3 NH4NO3, and (NH4)2SO4 inhibited this enzyme accumulation.
Secretion of VP and MnP by the investigated strain was comparatively low. Adding different carbon sources to the medium caused variation of VP activity from 0.24 U/L (medium with xylan) to 32.4 U/L (medium with mandarine peels). The results demonstrated that MnP activity was almost two-fold lower than VP activity on all of the used sources. The content of kazein acid and corn step liquor exerted the most favorable influence on the activity of VP and MnP. On the other hand, NH4NO3, NH4Cl, and (NH4)2SO4 were rather poor nitrogen sources for the production of these peroxidases.
In conclusion, it was shown that the synthesis of Lac, MnP, and VP by P. ostreatus 98 differed in various nutritional conditions. Among the different carbon and nitrogen sources, mandarine peels and peptone were optimal substrates for high production of the investigated enzymes.


Articles with similar content:

Influence of Selected Microelements on the Laccase and Peroxidases Production by Pleurotus eryngii (DC.: Fr.) Quel. in Submerged Cultures
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Yitzhak Hadar, Limor-Leibovitz Persky, Jelena Vukojević, Dana Friesem, Sonja Duletic-Lausevic, Mirjana Stajic
Bioconversion of Plant Raw Materials in Value-Added Products by Lentinus edodes (Berk.) Singer and Pleurotus spp.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George G. Songulashvili, Nana Aladashvili, Yitzhak Hadar, Michel Penninckx, Mikheil D. Asatiani, Eka Metreveli, Vladimir I. Elisashvili
Use of Agro-Industrial Waste for Production of Laccase and Manganese Peroxidase from White-Rot Basidiomycetes
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Yitzhak Hadar, Moti Rebhun
Effect of the Carbon Source and Inoculum Preparation Method on Laccase and Manganese Peroxidase Production in Submerged Cultivation by the Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
George G. Songulashvili, Yitzhak Hadar, Vladimir I. Elisashvili, Eviatar D. Nevo
Evaluation of Lignin-Modifying Enzyme Activity of Trametes spp. (Agaricomycetes) Isolated from Georgian Forests with an Emphasis on T. multicolor Biosynthetic Potential
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 10
Aza Kobakhidze, Eva Kachlishvili, Mikheil D. Asatiani, Vladimir I. Elisashvili