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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031849
pages 841-850

Review of Bioactive Molecules Production, Biomass, and Basidiomata of Shiitake Culinary-Medicinal Mushrooms, Lentinus edodes (Agaricomycetes)

Rigoberto Gaitán-Hernández
Biotechnology Resources Management Network, Institute of Ecology AC, Xalapa, Veracruz, Mexico
Damian López-Peña
Research Center for Food and Development AC, Hermosillo, Sonora, Mexico
Martín Esqueda
Research Center for Food and Development AC, Hermosillo, Sonora, Mexico
Aldo Gutiérrez
Research Center for Food and Development AC, Hermosillo, Sonora, Mexico


Lentinus edodes (shiitake) is a basidiomycete that has been consumed for more than 2000 years because of its nutritional value and health benefits. It has a low lipid content, high fiber content, and a considerable amount of proteins; it also contains B vitamins and minerals in addition to a wide range of functional metabolites including polysaccharides, polysaccharopeptides, lectins, and secondary metabolites with bioactivity, e.g., lentinan, a β-(1-3)-glucan with immunomodulatory activity, among others. Extracts and pure compounds of shiitake exhibit antibacterial, antifungal, cytostatic, antioxidant, anticancer, and immunomodulatory activity. Because of these attributes, different products derived from shiitake are on the market and are sold as dietary supplements. The traditional substrate for shiitake production is oak wood, yet the search for unconventional substrates has intensified over the past three decades. In particular, submerged cultivation of medicinal mushrooms has attracted great interest because it enables greater control of different fermentation factors to obtain products of interest. However, it is necessary to perform in vivo studies to determine the appropriate doses, side effects, and action spectrum of different bioactive compounds and fractions as well as to improve their production in liquid media and to potentiate their activity. We present an updated review of existing studies on the production of biomass and bioactive compounds of L. edodes in liquid culture and on solid fermentation for obtaining secondary mycelia and basidiomata.


  1. Wasser S. Shiitake (Lentinus edodes). In: Coates PM, Blackman MR, Cragg GM, Levine M, Moss J, White JD, editors. Encyclopedia of Dietary Supplements. New York: Marcel Dekker Inc., 2005, p. 653-64.

  2. Turlo J, Gutkowska B, Herold F, Krzyczkowski W, Blazewicz A, Kocjan R. Optimizing vitamin B12 biosynthesis by mycelial cultures of Lentinula edodes (Berk.) Pegl. Enzyme Microb Technol. 2008;43:369-74.

  3. Qaglarirmak N. The nutrients of exotic mushrooms (Lentinula edodes and Pleurotus species) and an estimated approach to the volatile compounds. Food Chem. 2007;105:1188-94.

  4. Neelam KPS, Mishra SK. Yield performance and element profiling of different strains of Lentinula edodes. In: Singh M, editor. Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products. New Delhi (India): Mushroom Society of India; 2014; pp. 365-68.

  5. Stamets P. Growing Gourmet and Medicinal Mushrooms. 3rd ed. Berkeley: Ten Speed Press, 2000.

  6. Chang S, Miles P. Cultivation, Nutritional Value, Medicinal Effect, and Environment Impact. Florida: CRC Press, 2004.

  7. Bisen PS, Baghel RK, Sanodiya BS, Thakur GS, Prasad GB. Lentinus edodes: a macrofungus with pharmacological activities. Curr Med Chem. 2010;17:2419-30.

  8. Kang MY, Rico CW, Lee SC. In vitro antioxidative and antimutagenic activities of oak mushroom (Lentinula edodes) and king oyster mushroom (Pleurotus eryngii) byproducts. Food Sci Biotechnol. 2012;21:167-73.

  9. Zhang Y, Li S, Wang X, Zhang L, Cheung P. Advances in lentinan: isolation, structure, chain conformation and bioactivities. Food Hydrocolloids. 2011;25:196-206.

  10. Royse DJ, Sanchez JE. Worldwide production of oyster mushrooms Pleurotus spp. with emphasis on Iberoamerican countries. In: Sanchez JE, Royse DJ, editors. La Biologia, el Cultivo y las Propiedades Nutricionales y Medicinales de las Setas Pleurotus spp. Tapachula (Mexico): El Colegio de la Frontera Sur, 2017, p. 17-25.

  11. Tan Q, Shang X, Zhang L, Li Y, Yu H, Zhou F, Zhang M, Song C. Lentinula edodes cultivation techniques and models in China. In: Singh M, editor. Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products. New Delhi, India: Mushroom Society of India; 2014; pp. 271-73.

  12. Przybylowicz P, Donoghue J. Shiitake Growers Handbook. The Art and Science of Mushroom Cultivation. Dubuque: Kendall/Hunt Pub. Co., 1990.

  13. Lin FC, Yang XM, Wang ZW. Cultivation of the black oak mushroom Lentinula edodes in China. Mushroom Sci. 2000;15:955-58.

  14. Royse DJ. Yield stimulation of shiitake by millet supplementation of wood chip substrate. In: Royse DJ, editor. Mushroom Biology and Mushroom Products. University Park, PA: Penn State University Press; 1996; pp. 277-83.

  15. Philippoussis A, Diamantopoulou P, Israilides C. Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinula edodes. Int Biodeterior Biodegradation. 2007;59:216-19.

  16. Gaitan-Hernandez R, Cortes N, Mata G. Improvement of yield of shiitake on wheat straw by use of supplemented spawn. Braz J Microbiol. 2014;45:467-74.

  17. Feng YL, Li WQ, Wu XQ, Cheng JW, Ma SY. Statistical optimization of media for mycelia growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies. Biochem Eng J. 2010;49:104-12.

  18. Lopez-Pena D, Gutierrez A, Esqueda, M. Cinetica de crecimiento y composition quimica del micelio de Lentinula edodes cultivado en medio hquido suplementado con extractos de madera de vid. Rev Mex Micol. 2013;37:51-59.

  19. Ishikawa NK, Megumi Kusuya MC, Dantas Vanetti MC. Antibacterial activity of Lentinula edodes grown in liquid medium. Braz J Microbiol. 2001;32:206-10.

  20. Hearst R, Nelson D, McCollum G, Millar BC, Maeda Y, Goldsmith CE, Rooney PJ, Loughrey A, Rao JR, Moore JE. An examination of antibacterial and antifungal properties of constituents of shiitake (Lentinula edodes) and oyster (Pleurotus ostreatus) mushrooms. Complement Ther Clin Pract. 2009;15:5-7.

  21. Kitzberger C, Smania A, Pedroza R, Ferreira S. Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J Food Eng. 2007;80:631-38.

  22. Rao JR, Millar BC, Moore JE. Antimicrobial properties of shiitake mushrooms (Lentinula edodes). Int J Antimicrob Agents. 2009;33:591-92.

  23. Ngai PHK, Ng TB. Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells. Life Sci. 2003;73:3363-74.

  24. Turlo J, Gutkowska B, Klimaszewska M, Kapusta C, Schneider K, Sikora M, Cieslak M, Julia Kazmierczak-Baranska J, Gorski A, Purchla S, Golas A. Selenium-enriched polysaccharide fraction isolated from mycelial culture of Lentinula edodes (Berk). Preliminary analysis of the structure and biological activity. In: Savoie JM, Foulongne-Oriol M, Largeteau M, Barroso G, editors. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products. Bordeaux, France: Institut National de la Recherche Agronomique; 2011; pp. 242-46.

  25. Enman J, Rova U, Berglund KA. Quantification of the bioactive compound eritadenine in selected strains of shiitake mushroom. J Agric Food Chem. 2007;55:1177-80.

  26. Chen J, Seviour R. Medicinal importance of fungal P-(1-3), (1-6)-glucans. Mycol Res. 2007;111:635-52.

  27. Suzuki H, Okubo A, Yamazaki S, Suzuki K, Mitsuya H, Toda S. Inhibition of the infectivity and cytopathic effect of human immunodeficiency virus by water-soluble lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM). Biochem Biophys Res Commun. 1989;160:367-73.

  28. Carbonero ER, Gracher AHP, Komura DL, Marcon R, Freitas CS, Baggio CH, Santos ARS, Torri G, Gorin PAJ, Iacomini M. Lentinus edodes heterogalactan: Antinociceptive and anti-inflammatory effects. Food Chem. 2008;111:531-37.

  29. Israilides C, Kletsas D, Arapoglou D, Philippoussis A, Pratsinis H, Ebringerova A, Hribalova V, Harding SE. In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes. Phytomedicine. 2008;15:512-19.

  30. Yap AT, Ng ML. An improved method for the isolation of lentinan from the edible and medicinal shiitake mushroom, Lentinus edodes (Berk.) Sing. (Agaricomycetideae). Int J Med Mushrooms. 2001;3:9-19.

  31. Hobbs CR. Medicinal value of Lentinus edodes (Berk.) Sing. (Agaricomycetideae). A literature review. Int J Med Mushrooms. 2000;2:287-302.

  32. Kojima H, Akaki J, Nakajima S, Kamei K, Tamesada M. Structural analysis of glicogen-ike polysaccharides having macrophage-activating activity in extracts of Lentinula edodes mycelia. J Nat Med. 2010;64:16-23.

  33. Sun B, Wakame K, Sato E, Nishioka H, Aruoma OI, Fuji H. The effect of active hexose correlated compound in modulating cytosine arabinose-induced hair loss, and 6-mercaptopurine- and methotrexate-induced liver injury in rodents. Cancer Epi-demiol. 2009;33:293-99.

  34. Kupfahl C, Geginat G, Hof H. Lentinan has a stimulatory effect on innate and adaptive immunity against murine Listeria monocytogenes infection. Int Immunopharmacol. 2006;6:686-96.

  35. Zaidman B-Z, Yassin M, Mahajna J, Wasser SP. Medicinal mushroom modulators of molecular targets as cancer therapeutics. Appl Microbiol Biotechnol. 2005;67:453-468.

  36. Coffelt SB, Lewis CE, Naldini L, Brown JM, Ferrara N, De Palma M. Elusive identities and overlapping phenotypes of proangiogenic myeloid cells in tumors. Am J Pathol. 2010;176:1564-76.

  37. Mantovani A. Molecular pathways linking inflammation and cancer. Curr Mol Med. 2010;10:369-73.

  38. Qian B, Pollard JW. Macrophage diversity enhances tumor progression and metastasis. Cell. 2010;141:39-51.

  39. Tsivileva OM, Pankratov AN, Nikitina VE. Extracellular protein production and morphogenesis of Lentinula edodes in submerged culture. Mycol Prog. 2010;9:157-67.

  40. G. Konska. Lectins in higher fungi (macromycetes)-their occurrence, physiological role, and biological activity. Int J Med Mushrooms. 2006;8(1):19-30.

  41. Taguchi T. Clinical trials on lentinan (polysaccharide). In: Yamamura Y, editor. Immunomodulation by microbial products and related synthetic compounds. New York: Elsevier; 1982; pp. 467-75.

  42. Mata JL, Mishra NT. Comparative study of worldwide species of genus Lentinus (=Lentinula, higher Basidiomycetes) based on linear mycelium growth. Int J Med Mushrooms. 2015;17:481-89.

  43. Gaitan-Hernandez R, Esqueda M, Gutierrez A, Sanchez A, Beltran-Garcia M, Mata G. Bioconversion of agrowastes by Lentinula edodes: the high potencial of viticulture residues. Appl Microbiol Biotechnol. 2006;71:432-39.

  44. Philippoussis AN, Diamantopoulou PA, Zervakis GI. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes. World J Microbiol Biotechnol. 2003;19:551-57.

  45. Royse DJ, Sanchez JE. Ground wheat straw as a substitute for portions of oak wood chips used in shiitake (Lentinula edodes) substrate formulae. Bioresource Technol. 2007;98:2137-41.

  46. Pire DG, Wright JE, Alberto E. Cultivation of shiitake using sawdust from widely available local woods in Argentina. Micol Apl Int. 2001;13:87-91.

  47. Gaitan-Hernandez R, Mata G. Cultivation of the edible mushroom Lentinula edodes (shiitake) in pasteurized wheat straw-alternative use of geothermal energy in Mexico. Eng Life Sci. 2004;4:363-67.

  48. Gaitan-Hernandez R, Esqueda M, Gutierrez A, Beltran-Garcia M. Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes. Braz J Microbiol. 2011;42:30-40.

  49. Gaitan-Hernandez R, Barradas Zavaleta M, Aquino-Bolanos EN. Productivity, physicochemical changes, and antioxidant activity of shiitake culinary-medicinal mushroom Lentinus edodes (Agaricomycetes) cultivated on lignocellulosic residues. Int J Med Mushrooms. 2017;19:1041-1052.

  50. Gregori A, Kretschmer N, Wagner S, Boechzelt H, Klinar D, Bauer R, Pohleven F. Influence of olive oil press cakes on shiitake culinary-medicinal mushroom, Lentinus edodes (Berk.) Singer (higher Basidiomycetes) fruiting bodies production and effect of their crude polysaccharides on CCRF-CEM cell proliferation. Int J Med Mushrooms. 2012;14:419-24.

  51. Villegas V, Milena A, Arredondo C. Evaluacion de la produccion del hongo Lentinula edodes Pegler en bloques sinteticos a base de residuos agroindustriales. Ing Cienc. 2007;3(6):23-39.

  52. Ozjelik E, Pek^en A. Hazelnut husk as a substrate for the cultivation of shiitake mushroom (Lentinula edodes). Bioresource Technol. 2007;98:2652-58.

  53. Fung YW, Fung TW, Franco M. Evaluation of different Colombian agroindustrial wastes as substrates for the growth production of Lentinula edodes (Berk.) Pegler (shiitake). In: Tan Q, Zhang J, Chen M, Cao H, Buswell JA, editors. Mushroom Biology and Mushroom Products. Shanghai, China: Shanghai Academy of Agriculture Sciences; 2005; pp. 285-290.

  54. Soto-Velazco C, Fausto S, Guzman-Davalos L. Cultivation of the mushrooms Lentinus boryanus and L. edodes on a mixture of maguey tequilero bagasse and sugarcane bagasse. Afr J Mycol Biotechnol. 1995;3:115-20.

  55. Jaramillo C, Rodriguez N, Chang ST. Cultivation of shiitake on coffee waste. In: Romaine CP, Keil CB, Rinker DL, Royse DJ, editors. Science and Cultivation of Edible and Medicinal Fungi. State College, PA: Penn State University Press, 2004, p. 307-11.

  56. Cabrera R, Lopez-Pena D, Asaff A, Esqueda M, Valenzuela-Soto E. Bioavailability of compounds susceptible to enzymatic oxidation enhances growth of shiitake medicinal mushroom, Lentinus edodes (Agaricomycetes), in solid-state fermentation with vineyard prunings. Int J Med Mushrooms. 2018;20:291-303.

  57. Elisashvili V, Kachlishvili E, Asatiani M. Shiitake medicinal mushroom, Lentinus edodes (higher Basidiomycetes) productivity and lignocellulolytic enzyme profiles during wheat straw and tree leaf bioconversion. Int J Med Mushrooms. 2015;17:77-86.

  58. Enman J, Hodge D, Rova U, Berglund KA. Production of the bioactive compound eritadenine by submerged cultivation of shiitake (Lentinus edodes) mycelia. J Agric Food Chem. 2008;56:2609-12.

  59. Jiao YC, Chen QH, Zhou JS, Zhang HF, Chen HY. Improvement of exo-polysaccharides production and modeling kinetics by Armillaria luteo-virens Sacc. in submerged cultivation. Food Sci Technol. 2008;41:1694-1700.

  60. Luo J, Liu J, Ke C, Qiao D, Ye H, Sun Y, Zeng XX. Optimization of the medium composition for the production of exopoly-saccharides from Phellinus baumii Pilat in submerged culture and the immuno-stimulating activity of exopolysaccharides. Carbohydr Polym. 2009;78:409-15.

  61. Shivani S, Khanna PK. Statistical optimization of process variables for the production of mycelial biomass and polysaccharides from Lentinus edodes. In: Zhang J, Wang H, Chen M, editors. Proceedings of the 18th Congress Int Soc Mushroom Sci. Beijing, China: China Agriculture Press; 2012; pp. 592-600.

  62. Babitskaya VG, Bisko NA, Scherba VV, Mitropolskaya NY, Puchkova TA. Some physiological aspects of the submerged cultivation of culinary-medicinal shiitake mushroom Lentinus edodes (Berk.) Singer (Agaricomycetideae). Int J Med Mushrooms. 2004;6:369-74.

  63. de Souza L, dos Santos AC, Souza T, Figueiredo AL, Simas MF. The influence of different submerged cultivation conditions on mycelial biomass and protease production by Lentinus citrinus Walleyn et Rammeloo DPUA 1535 (Agaricomycetidae). Int J Med Mushrooms. 2011;13:185-92.

  64. Elisashvili V. Submerged cultivation of medicinal mushrooms: bioprocesses and products (Review). Int J Med Mushrooms. 2012;14:211-39.

  65. Tsivileva OM, Nikitina VE, Garibova LV. Effect of culture medium composition on the activity of extracellular lectins of Lentinus edodes. Appl Biochem Microbiol. 2005;41:174-176.

  66. Nikitina VE, Tsivileva OM, Pankratov AN, Bychkov NA. Lentinula edodes biotechnology-from lentinan to lectins. Food Technol Biotechnol. 2007;45:230-37.

  67. Elisashvili V, Wasser S, Tan KK, Chichua D, Kachlishvili E. Extracellular polysaccharide production by culinary-medicinal shiitake mushroom Lentinus edodes (Berk.) Singer and Pleurotus (Fr.) P. Karst. Species depending on carbon and nitrogen source. Int J Med Mushrooms. 2004;6:165-72.

  68. Harris-Valle C, Esqueda M, Sanchez A, Beltran-Garcia M, Valenzuela-Soto E. Polar vineyard pruning extracts increase the activity of the main ligninolytic enzymes in Lentinula edodes cultures. Can J Microbiol. 2007;53:1150-57.

  69. Leatham GF, Griffin TJ. Adapting liquid spawn Lentinus edodes to oak wood. Appl Microbiol Biotechnol. 1984;20:360-63.

  70. Kawai G, Kobayashi H, Fukushima Y, Ohsaki K. Effect of liquid mycelial culture used as a spawn on sawdust cultivation of shiitake (Lentinula edodes). Mycoscience. 1996;37:201-7.

  71. Cavallazzi JRP, Kasuya CM, Soares MA. Screening of inducers for laccase production by Lentinula edodes in liquid medium. Braz J Microbiol. 2005;36:383-87.

  72. D'Annibale A, Casa R, Pieruccetti F, Ricci M, Marabottini R. Lentinula edodes removes phenols from olive-mill waste-water: impact on durum wheat (Triticum durum Desf.) germinability. Chemosphere. 2004;54:887-94.

  73. Lakhtar H, Ismaili-Alaoui M, Philippoussis A, Perraud-Gaime I, Roussos S. Screening of strains of Lentinula edodes grown on model olive mill wastewater in solid and liquid state culture for polyphenol biodegradation. Int Biodeterior Biodegradation. 2010;64:167-72.

  74. Tomati U, Belardinelli M, Galli E, Iori V, Capitani D, Mannina L, Viel S, Segreb A. NMR characterization of the polysaccharide fraction from Lentinula edodes grown on olive mill waste waters. Carbohydr Res. 2004;339:1129-34.

  75. Lee S, Bae H, Kim N, Hwang S. Optimization of growth conditions of Lentinus edodes mycelium on corn proccessing waste using responce surface analysis. J Biosci Bioeng. 2008;105(2):161-63.

  76. Freire-Moran L, Aronsson B, Manz C, Gyssens IC, So AD, Monnet DL, Otto Cars, Norrby R, Powell M. Lutsar I, Bocsan I, Giamarellou H. Critical shortage of new antibiotics in development against multidrug-resistant bacteria-time to react is now. Drug Resist Updat. 2011;14:118-24.

  77. Wasser SP. Medicinal mushroom science: history, current sttaus, future trends, and unsolved problems. Int J Med Mushrooms. 2010;12(1):1-16.

  78. Reverberi M, Di Mario F, Tomati U. P-glucan synthase induction in mushrooms grown on olive mill wastewaters. Appl Microbiol Biotechnol. 2004;66:217-25.

  79. Molleken H, Nitschke J, King HW, Altenbach HJ. Enzymatic extraction, quantification and structural characterization of P-glucans from specific mushrooms (fruitbodies and mycelia cultures). In: Zhang J, Wang H, Chen M, editors. Proceedings of the 18th Congress of the Int Soc Mushroom Sci. Beijing, China: China Agriculture Press; 2012; pp. 601-10.

  80. Zhu F, Du B, Bian Z, Xu B. P-glucans from edible and medicinal mushrooms: characteristics physicochemical and biological activities. J Food Comp Anal. 2015;41:165-73.