Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
Antioxidant and Neuroprotector Influence of Endo-Polyphenol Extract from Magnesium Acetate Multi-Stage Addition in the Oak Bracket Medicinal Mushroom, Phellinus baumii (Agaricomycetes)
RESUMO
The objective of this study was to explore the effect of magnesium acetate (MA) addition on the endo-polyphenol yield by Phellinus baumii and establish a feasible additive strategy. The optimal three-point MA addition strategy (0.05 g/L concentration of MA added at 0 h and 6 h, 0.9 g/L concentration of MA added at 12 h) was employed to obtain maximum endo-polyphenol yield. The maximum endo-polyphenol production was reached at 1.22 g/L, which was 1.39-fold higher than that of the control. Additionally, the endo-polyphenol showed stronger antioxidant activity in vitro compared with the control, including DPPH· scavenging capacity (78.76%) and Trolox equivalent antioxidant capacity (TEAC) (32.28 μmol Trolox/g sample). HPLC analysis showed that the endo-polyphenol production of the crude ethanol extracts was significantly higher than that of the control. Hispidin was isolated and identified from the ethanol extract of the culture mycelia from Ph. baumii with the three-point MA addition strategy. Hispidin showed a strong ability to scavenge DPPH free radicals and TEAC, equivalent to positive (vitamin C) value of 89.41% and 75.98%, respectively. Furthermore, hispidin protected H2O2-induced PC12 cells injured by decreased oxidative stress level. These results indicated that the MA multi-stage addition strategy was dependable, and could be used to develop new natural antioxidants for foods or medicines.
-
Shao Q, Yang Y, Li T, Feng J, Liu Y, Yan M, Zhu L, Tang C. Biological activities of ethanol extracts of Phellinus baumii (higher Basidiomycetes) obtained by different fermentation methods. Int J Med Mushrooms. 2015;17(4):361-9.
-
Lee S, Lee D, Jang TS, Kang KS, Nam JW, Lee HJ, Kim KH. Anti-inflammatory phenolic metabolites from the edible fungus Phellinus baumii in LPS-stimulated RAW264.7 cells. Molecules. 2017;22(10):pii:E1583.
-
Liu MM, Zeng P, Li XT, Shi LG. Antitumor and immunomodulation activities of polysaccharide from Phellinus baumii. Int J Biol Macromol. 2016;91:1199-1205.
-
Ayala-Zavala JF, Perez-Carlon JJ, Esqueda M, Gonzalez-Aguilar GA, Leyva JM, Cruz-Valenzuela MR, Moctezuma E. Polar fractionation affects the antioxidant properties of methanolic extracts from species of genus Phellinus Quel. (higher Basidiomycetes). Int J Med Mushrooms. 2012;14(6):563-73.
-
Kovacs B, Zomborszki ZP, Orban-Gyapai O, Csupor-Loffler B, Liktor-Busa E, Lazar A, Papp V, Urban E, Hohmann J, Vanyolos A. Investigation of antimicrobial, antioxidant, and xanthine oxidase-inhibitory activities of Phellinus (Agaricomycetes) mushroom species native to Central Europe. Int J Med Mushrooms. 2017;19(5):387-94.
-
Gao W, Wang W, Sun W, Wang M, Zhang N, Yu S. Antitumor and immunomodulating activities of six Phellinus igniarius polysaccharides of different origins. Exp Ther Med. 2017;14(5):4627-32.
-
Zhao C, Liao Z, Wu X, Liu Y, Liu X, Lin Z, Huang Y, Liu B. Isolation, purification, and structural features of a polysaccharide from Phellinus linteus and its hypoglycemic effect in alloxan-induced diabetic mice. J Food Sci. 2014;79(5):H1002-10.
-
Wei S, Helsper JPFG, van Griensven LJLD. Phenolic compounds present in medicinal mushroom extracts generate reactive oxygen species in human cells in vitro. Int J Med Mushrooms. 2008;10(1):1-13.
-
Song TY, Yang NC, Chen CL, Thi TLV. Protective effects and possible mechanisms of ergothioneine and hispidin against methylglyoxal-induced injuries in rat pheochromocytoma cells. Oxid Med Cell Longev. 2017;2017:4824371.
-
Yi YJ, Lee IK, Lee SM, Yun BS. An antioxidant davallialactone from Phellinus baumii enhances sperm penetration on in vitro fertilization of pigs. Mycobiology. 2016;44(1):54-57.
-
El Hassane A, Shah SA, Hassan NB, El Moussaoui N, Ahmad R, Zulkefeli M, Weber JF. Antioxidant activity of hispidin oligomers from medicinal fungi: a DFT study. Molecules. 2014;19(3):3489-507.
-
Chen W, Shen Y, Su H, Zheng X. Hispidin derived from Phellinus linteus affords protection against acrylamide-induced oxidative stress in Caco-2 cells. Chem Biol Interact. 2014;219:83-89.
-
Zhu H, Sun S, Zhang S. Enhanced production of total flavones and exopolysaccharides via Vitreoscilla hemoglobin biosynthesis in Phellinus igniarius. Bioresour Technol. 2011;102(2):1747-51.
-
Chen H, Tian T, Miao H, Zhao YY. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus: a review. Fitoterapia. 2016;113:6-26.
-
Wang Z, Quan Y, Zhou F. Optimization of medium composition for exopolysaccharide production by Phellinus nigricans. Carbohydr Polym. 2014;105:200-6.
-
Xu X, Xu Z, Shi S, Lin M. Lignocellulose degradation patterns, structural changes, and enzyme secretion by Inonotus obliquus on straw biomass under submerged fermentation. Bioresour Technol. 2017;241:415-23.
-
Zheng W, Liu Y, Pan S, Yuan W, Dai Y, Wei J. Involvements of S-nitrosylation and denitrosylation in the production of polyphenols by Inonotus obliquus. Appl Microbiol Biotechnol. 2011;90(5):1763-72.
-
Ma XK, Zhang H, Peterson EC, Chen L. Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies. Carbohydr Polym. 2014;107:214-20.
-
Li T, Yang Y, Liu Y, Zhou S, Yan MQ, Wu D, Zhang J, Tang C. Physicochemical characteristics and biological activities of polysaccharide fractions from Phellinus baumii cultured with different methods. Int J Biol Macromol. 2015;81:1082-88.
-
Ma XK, Li L, Peterson EC, Ruan T, Duan X. The influence of naphthaleneacetic acid (NAA) and coumarin on flavonoid production by fungus Phellinus sp.: modeling of production kinetic profiles. Appl Microbiol Biotechnol. 2015;99(22):9417-26.
-
Gao XX, Tuli GE, Yao Q, Cheng XH, Laio J, Yang SD, Li WH, Liu Y, Huang H. Sericulture & Agri-Food Research Institute GAAS. Methods for enhancing flavonoid yield in liquid fermentation. 2009. China patent 201310638380.7. 2013 Dec 4.
-
Wang Y, Yu JX, Zhang CL, Li P, Zhao YS, Zhao MH, Zhou PG. Influence of flavonoids from Phellinus igniarius on sturgeon caviar: antioxidant effect and sensory characteristics. Food Chem. 2012;131(1):206-10.
-
Konwarh R, Pramanik S, Kalita D, Mahanta CL, Karak N. Ultrasonication-a complementary 'green chemistry' tool to bio-catalysis: a laboratory-scale study of lycopene extraction. Ultrason Sonochem. 2012;19(2):292-99.
-
Zhang H, Ma H, Liu W, Pei J, Wang Z, Zhou H, Yan J. Ultrasound enhanced production and antioxidant activity of polysaccharides from mycelial fermentation of Phellinus igniarius. Carbohydr Polym. 2014;113:380-87.
-
Yousfi M, Djeridane A, Bombarda I, Duhem B, Gaydou EM. Isolation and characterization of a new hispolone derivative from antioxidant extracts of Pistacia atlantica. Phytother Res. 2009;23(9):1237-42.
-
Yang Y, Hu J, Liu Y, Feng N, Chen H, Tang Q, Ye L, Zhang JS. Antioxidant and cytotoxic activities of ethanolic extracts and isolated fractions of species of the genus Phellinus Quel. (Aphyllophoromycetidae). Int J Med Mushrooms. 2011;13(2):145-52.
-
Smolskaite L, Slapsyte G, Mierauskiene J, Dedonyte V, Venskutonis PR. Antioxidant and genotoxic properties of hispidin isolated from the velvet-top mushroom, Phaeolus schweinitzii (Agaricomycetes). Int J Med Mushrooms. 2017;19(11):967-80.
-
Gornostai TG, Borovskii GG, Kashchenko NI, Olennikov DN. Phenolic compounds of inonotus rheades (Agaricomycetes) mycelium: RP-UPLC-DAD-ESI/MS profile and effect of light wavelength on styrylpyrone content. Int J Med Mushrooms. 2018;20(7):637-45.
-
Lee IK, Yun BS. Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J Antibiot (Tokyo). 2011;64(5):349-59.
-
Jung JY, Lee IK, Seok SJ, Lee HJ, Kim YH, Yun BS. Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus. J Appl Microbiol. 2008;104(6):1824-32.
-
Li N, Zhao L, Ng TB, Wong JH, Yan Y, Shi Z, Liu F. Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer. Appl Microbiol Biotechnol. 2015;99(18):7569-77.
-
Vyas P, Rahi P, Chadha BS, Gulati A. Statistical optimization of medium components for mass production of plant growth-promoting microbial inoculant pseudomonas trivialis BIHB 745 (MTCC5336). Indian J Microbiol. 2014;54(2):239-41.
-
Tian XM, Dai YC, Song AR, Xu K, Ng LT. Optimization of liquid fermentation medium for production of inonotus sanghuang (higher Basidiomycetes) mycelia and evaluation of their mycochemical contents and antioxidant activities. Int J Med Mushrooms. 2015;17(7):681-91.
-
Ma XK, Zhang H, Fam H. Influence of rutin, FeSO4, Tween 80, aspartate and complex vitamins on synthesis of fungal exo-polysaccharide. Carbohydr Polym. 2013;92(2):1188-96.
-
Xu X, Quan L, Shen M. Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus. Int J Biol Macromol. 2015;77:143-50.
-
Zheng W, Zhang M, Zhao Y, Wang Y, Miao K, Wei Z. Accumulation of antioxidant phenolic constituents in submerged cultures of Inonotus obliquus. Bioresour Technol. 2009;100(3):1327-35.
-
Loake GJ, Choudhary AD, Harrison MJ, Mavandad M, Lamb CJ, Dixon RA. Phenylpropanoid pathway intermediates regulate transient expression of a chalcone synthase gene promoter. Plant Cell. 1991;3(8):829-40.
-
Hoefgen R, Nikiforova VJ. Metabolomics integrated with transcriptomics: assessing systems response to sulfur-deficiency stress. Physiol Plant. 2008;132(2):190-98.
-
Rizvi A, Yousf S, Balakrishnan K, Dubey HK, Mande SC, Chugh J, Banerjee S. Metabolomics studies to decipher stress responses in Mycobacterium smegmatis point to a putative pathway of methylated amines biosynthesis. J Bacteriol. 2019;201(15):e00707-18.
-
Yang Kai, Zhang Su, Geng Yan, Tian Baoming, Cai Ming, Guan Rongfa, Li Yougui, Ye Bangwei, Sun Peilong, Anti-Inflammatory Properties In Vitro and Hypoglycaemic Effects of Phenolics from Cultivated Fruit Body of Phellinus baumii in Type 2 Diabetic Mice, Molecules, 26, 8, 2021. Crossref
-
Badalyan Susanna M., Rapior Sylvie, The Neurotrophic and Neuroprotective Potential of Macrofungi, in Medicinal Herbs and Fungi, 2021. Crossref
-
Li I-Chen, Chang Fang-Chia, Kuo Ching-Chuan, Chu Hsin-Tung, Li Tsung-Ju, Chen Chin-Chu, Pilot Study: Nutritional and Preclinical Safety Investigation of Fermented Hispidin-Enriched Sanghuangporus sanghuang Mycelia: A Promising Functional Food Material to Improve Sleep, Frontiers in Nutrition, 8, 2022. Crossref