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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i3.70
pages 279-286

Anti-Human Papillomavirus (HPV) 16 E6 Activity of Ling Zhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Extracts

Li Kuan Lai
Institute of Biological Sciences, Faculty of Science, University Malaya, Kuala Lumpur, Malaysia
Nurhayati Zainal Abidin
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Noorlidah Abdullah
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Vikineswary Sabaratnam
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

RESUMO

Cancer of the cervix ranks second in the demographics of cancers affecting women worldwide. Human papillomavirus (HPV) type 16 is one of the major causative agents for cervical cancer. The E6 region of the HPV 16 genome encodes for an oncoprotein responsible for the pathogenesis of the disease. The E6 oncoprotein is abundant in cervical cancer and cervical cancer-derived cell lines. Mushrooms of the genus Ganoderma have long been used traditionally as an immunomodulating and antitumor agent, with recent studies validating its use in chemotherapeutics. Due to the importance of the E6 oncoprotein in the carcinogenesis of cervical cancer, this study was undertaken to screen the crude dichloromethane, ethanol, water, and polysaccharide extracts of Ganoderma lucidum for the ability to suppress the expression of HPV 16 E6 and to evaluate secondary metabolite classes present in the active crude extract. CaSki cells were treated with the crude extracts and were then subjected to immunocytochemistry protocols. All crude extracts of G. lucidum presented HPV 16 E6 suppression, with the dichloromethane extract being the most active compared to the other crude extracts. The crude dichloromethane extract was then subjected to thin-layer chromatography to evaluate the secondary metabolite classes present. The present study shows that the crude dichloromethane extract of G. lucidum possesses flavonoids, terpenoids, phenolics, and alkaloids. Further isolation work on the dichloromethane extract of G. lucidum could direct us to lead compounds with promising anti-HPV 16 E6 activity.


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