Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i10.80
pages 987-996

Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes)

Xiulian Yin
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China
Qinghong You
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China; Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals & Oils, Nanjing University of Finance and Economics, Nanjing, China
Xinghai Zhou
School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, China

RESUMO

Agaricus bisporus polysaccharides (ABP) were extracted by complex enzyme-assisted extraction methodology. The following were optimal conditions for the extraction of crude ABP: complex enzyme amount, 2.2%; temperature, 62°C; time, 3 h; and pH, 4. Under these conditions, the experimental yield of crude ABP was 6.87%. The crude ABP was purified by diethylaminoethyl-cellulose 52 chromatography and Sephadex G-100 chromatography, and one fraction−namely, ABP-1−was produced. The ABP-1 contained 93.67% carbohydrate, 1.46% protein, and 0.62% uronic acid. The constituent monosaccharides were predominantly glucose, galactose, mannose, and xylose. The antioxidant activities of ABP-1 were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, its ferric-reducing activity power, and the reducing power assay. At a concentration of 1.2 mg/mL, ABP-1 seemed to possess good free radical scavenging activity, with a scavenging value of about 56%. The results indicate that ABP-1 has good antioxidant activity.


Articles with similar content:

Extraction, Antimicrobial, and Antioxidant Activities of Crude Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Peilong Sun, Yang Lin, Han-hua Liang, Yin-long Luo, Ming Cai
Purification, Composition Analysis, and Antioxidant Activity of Exopolysaccharides from Mycelial Culture of Paecilomyces cicadae (Miq.) Samson (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Xueqian Wu, Junwen Cheng, Xinyan Lu, Haibo Li, Liang He
Antioxidant Activity of Submerged Cultured Mycelium Extracts of Higher Basidiomycetes Mushrooms
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 2
Abraham Reznick, Mikheil D. Asatiani, Vladimir I. Elisashvili, Eviatar D. Nevo
Chemical Composition and Medicinal Value of the New Ganoderma tsugae var. jannieae CBS-120304 Medicinal Higher Basidiomycete Mushroom
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 8
Solomon P. Wasser, Lital E. Sharvit, Mikheil D. Asatiani, Gayane S. Barseghyan, Jannie Siew Lee Chan, Beny Trabelcy
A Comparative Study of the Antimicrobial, Antioxidant, and Cytotoxic Activities of Methanol Extracts from Fruit Bodies and Fermented Mycelia of Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 5
Tao Yang, Caihong Dong, Tiantian Lian