Inscrição na biblioteca: Guest
International Journal of Medicinal Mushrooms

Publicou 12 edições por ano

ISSN Imprimir: 1521-9437

ISSN On-line: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

Identification of Reference Genes and Analysis of Heat Shock Protein Gene Expression in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, after Exposure to Heat Stress

Volume 19, Edição 11, 2017, pp. 1029-1040
DOI: 10.1615/IntJMedMushrooms.2017024487
Get accessGet access

RESUMO

Ganoderma lucidum has been considered an emerging model species for studying how environmental factors regulate the growth, development, and secondary metabolism of Basidiomycetes. Heat stress, which is one of the most important environmental abiotic stresses, seriously affects the growth, development, and yield of microorganisms. Understanding the response to heat stress has gradually become a hotspot in microorganism research. But suitable reference genes for expression analysis under heat stress have not been reported in G. lucidum. In this study, we systematically identified 11 candidate reference genes that were measured using reverse transcriptase quantitative polymerase chain reaction, and the gene expression stability was analyzed under heat stress conditions using geNorm and NormFinder. The results show that 5 reference genes−CYP and TIF, followed by UCE2, ACTIN, and UBQ1−are the most stable genes under our experimental conditions. Moreover, the relative expression levels of 3 heat stress response genes (hsp17.4, hsp70, and hsp90) were analyzed under heat stress conditions with different normalization strategies. The results show that use of a gene with unstable expression (SAND) as the reference gene leads to biased data and misinterpretations of the target gene expression level under heat stress.

CITADO POR
  1. Gao Shuangshuang, Wang Gangzheng, Huang Zhicheng, Lei Xiaoyu, Bian Yinbing, Liu Ying, Huang Wen, Selection of Reference Genes for qRT-PCR Analysis in Lentinula edodes after Hot-Air Drying, Molecules, 24, 1, 2018. Crossref

  2. Hu Yanru, Xu Wenzhao, Hu Shishan, Lian Lingdan, Zhu Jing, Ren Ang, Shi Liang, Zhao Ming Wen, Glsnf1-mediated metabolic rearrangement participates in coping with heat stress and influencing secondary metabolism in Ganoderma lucidum, Free Radical Biology and Medicine, 147, 2020. Crossref

  3. Tian Jia-Long, Ren Ang, Wang Ting, Zhu Jing, Hu Yan-Ru, Shi Liang, Yu Han-Shou, Zhao Ming-Wen, Hydrogen sulfide, a novel small molecule signalling agent, participates in the regulation of ganoderic acids biosynthesis induced by heat stress in Ganoderma lucidum, Fungal Genetics and Biology, 130, 2019. Crossref

  4. Xia Jia-le, Wu Chen-Gao, Ren Ang, Hu Yan-ru, Wang Sheng-li, Han Xiao-fei, Shi Liang, Zhu Jing, Zhao Ming-wen, Putrescine regulates nitric oxide accumulation in Ganoderma lucidum partly by influencing cellular glutamine levels under heat stress, Microbiological Research, 239, 2020. Crossref

  5. Wang Ting, Wang Yihong, Chen Chen, Ren Ang, Yu Hanshou, Zhao Mingwen, Effect of the heme oxygenase gene on mycelial growth and polysaccharide synthesis in Ganoderma lucidum , Journal of Basic Microbiology, 61, 3, 2021. Crossref

  6. Tao Yongxin, Han Xiaofei, Ren Ang, Li Jian, Song Hanbing, Xie Baogui, Zhao Mingwen, Heat stress promotes the conversion of putrescine to spermidine and plays an important role in regulating ganoderic acid biosynthesis in Ganoderma lucidum, Applied Microbiology and Biotechnology, 105, 12, 2021. Crossref

  7. Yang Yayong, Xu Xinyu, Jing Zhuohan, Ye Jun, Li Hui, Li Xiaoyu, Shi Lei, Chen Mengyu, Wang Tengyun, Xie Baogui, Tao Yongxin, Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi, Journal of Fungi, 8, 9, 2022. Crossref

1272 Visualizações do artigo 33 downloads de artigos Métricas
1272 VISUALIZAÇÕES 33 TRANSFERÊNCIAS 7 Crossref CITAÇÕES Google
Scholar
CITAÇÕES

Artigos com conteúdo semelhante:

Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes) International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 3
Yanquan Lin, Lu Ma, Xiaoling Jiang, Chi Yang, Zhenghe Ying, Donglai Xiao
Selection and Validation of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Studies in Mossy Maze Polypore, Cerrena unicolor (Higher Basidiomycetes) International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 2
Qi Lin, Jie Yang, Xiuyun Ye, Juan Lin
Biosynthesis, Gene Expression, and Pharmacological Properties of Triterpenoids of Ganoderma Species (Agaricomycetes): A Review International Journal of Medicinal Mushrooms, Vol.24, 2022, issue 6
Damian López-Peña, Heriberto Torres-Moreno, Aldo Gutiérrez, Rigoberto Gaitán-Hernández, Lucia T. Angulo-Sanchez, Martín Esqueda
Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes) International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 6
Hong Gao, Chao-Min Yin, De-Fang Shi, Xiu-Zhi Fan, Fen Yao
Polysaccharide Elicitors Affect the Yield, Polysaccharide Synthase and Antibacterial Activity of Intracellular Polysaccharides from Submerged Culture of Cordyceps milifaris (Ascomycetes) International Journal of Medicinal Mushrooms, Vol.25, 2023, issue 2
Kai Ding, Chunchao Han, Yongxia Wang
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa Políticas de preços e assinaturas Begell House Contato Language English 中文 Русский Português German French Spain