Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i2.60
pages 161-168

Development of an Expression Plasmid and its Use in Genetic Manipulation of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes)

Xuya Yu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
Sen-Lin Ji
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
Yi-Long He
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
Meng-Fei Ren
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
Jun-Wei Xu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China

RESUMO

We report the construction of a plasmid, pJW-EXP, designed for the expression of homologous and heterologous genes in Ganoderma lucidum. pJW-EXP was generated from the plasmid pMD19-T by inserting the G. lucidum glyceraldehyde-3-phosphate dehydrogenase gene promoter, the G. lucidum iron-sulfur protein subunit of succinate dehydrogenase gene terminator and the homologous carboxin-resistance gene as selection marker. This expression plasmid can be efficiently transformed into Ganoderma through polyethylene glycol-mediated protoplast transformation. Southern blot analysis showed that most of the integrated DNA appeared as multiple copies in the genome. The applicability of the constructed plasmid was tested by expression of the truncated G. lucidum 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene that encodes the catalytic domain of HMGR. Overexpression of the truncated HMGR gene, which is a key gene in the biosynthetic pathway of the antitumor compounds, ganoderic acids, increased the transcription of the HMGR gene and enhanced ganoderic acid accumulation. pJW-EXP can serve as a useful tool in the genetic improvement and metabolic engineering of Ganoderma.


Articles with similar content:

Molecular Characterization and Antioxidant Potential of Three Wild Culinary-Medicinal Mushrooms from Tripura, Northeast India
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 1
Panna Das, Santa Ram Joshi, Aparajita Roy Das, Ajay Krishna Saha, Madhusmita Borthakur
Downregulation of Ethylene Production Increases Mycelial Growth and Primordia Formation in the Button Culinary-Medicinal Mushroom, Agaricus bisporus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Chaohui Shen, Liyou Qiu, Jinwen Shen, Andong Song, Tao Huang, Yuancheng Qi, Chaohui Zhang, Yuncan Ai, Xiaoting Wang
A Common Regulator of Cold and Radiation Response in Escherichia coli
Journal of Environmental Pathology, Toxicology and Oncology, Vol.20, 2001, issue 1
Vibhav R. Sanzgiri, Suhas Mangoli, Suresh K. Mahajan
Agrobacterium tumefaciens-Mediated Transformation of the King Tuber Medicinal Mushroom Lentinus tuber-regium (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Liguo Chen, Aimin Ma, Yue Chen, Liesheng Zheng, Hanyu Zhu, Dongmei Liu
Enzyme Preparations from Higher Basidiomycetes Mushrooms for Making Polymer Materials with Thromboresistant Features
International Journal of Medicinal Mushrooms, Vol.3, 2001, issue 1
Svetlana P. Novikova