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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v1.i1.80
pages 93-103

Cultural Study and llludin S Production of Medicinal Mushroom Omphalotus olearius (DC.Fr.) Fay. (Agaricales s.l.) from Israel

Alexander L. Weis
international Myko Biologies, Inc., Texas Research Park, 14785 Omicron Dr., Suite 104, San Antonio, TX, 78245, U.S.A.
Elvira F. Solomko
M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Tereshchenkivska Str. 2, GSP, 01601, Ukraine
Asja S. Buchalo
M. G. Kholodny Institute of Botany National Academy of Sciences of Ukraine, Tereshchenovskaya Str., 2, Kiev 01601, Ukraine
Solomon P. Wasser
N.G. Kholodny Institute of Botany, NAS of Ukraine, 2 Tereshchenkovskaya Str., Kiev 01004, Ukraine; International Center for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel
Nadezda Yu. Mitropolskaya
M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereschenkivska Str. 2, Kiev, 252601, Ukraine
Andrey Ph. Grigansky
M.G.Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska Str. 2, Kiev, 252001, Ukraine
Elena L. Gorovits
International Myko Biologies, Inc., 14785 Omicron Dr., Suite 104, San Antonio, TX, 78245, USA

RESUMO

The morphological and cultural characteristics of vegetative mycelium of 11 investigated strains of Omphalotus olearius (DC.:Fr.) Fay. (Omphalotaceae, Agaricales s.l.) were studied. The hyphae were found to have numerous clamps and formations of arthroconidia and crystals. The growth rate of mycelium (to 7.5 mm/day) on the surface varied depending on media composition. Production of orange-red pigment and the high activity of gelatinase, urease, cellulase, and β-glucosidase was observed in pure cultures. Optimum temperature was developed for growth appeared to be 28 °C, with an initial pH of complex media between 4.0 and 4.5. A methodical scheme for obtaining standard liquid inoculum useful for physiological and biochemical investigations of O. olearius. Illudin S content in liquid broth under submerged cultivation for all strains was determined. Depending on the strain, the content of illudin S was between 0.81 and 3.1 mg/ml. Strains 162, 167 and 169 have been selected for optimization of illudin S production.


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