Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
A Novel Technique for Rejuvenation of Degenerated Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes), a Valued Traditional Chinese Medicine
RESUMO
Cordyceps militaris has been used in traditional Chinese medicine for many years, but its frequent degeneration during continuous maintenance in culture can lead to substantial commercial losses. In this study, a degenerated strain of C. militaris was obtained by subculturing a wild-type strain through 10 successive subcultures. The relative abundance of the 2 mating types seems to be out of balance in the degenerated strain. By cross-mating 4 single-ascospore isolates (2 for MAT 1-1 and 2 for MAT 1-2) from the degenerated strain, we were able to restore fruiting body production to wild-type levels. The rejuvenated strain not only produced well-developed fruiting bodies but also accumulated more cordycepin and adenosine than either the original wild-type strain or the degenerated strain. These new characteristics remained stable after 4 successive transfers, which indicates that the method used to rejuvenate the degenerated strain in this study is an effective approach.
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Feng Yu-jie, Zhu Yun, Li Yong-mei, Li Jin, Sun Yan-fei, Shen Hai-tao, Wang Ai-ying, Lin Zhong-ping, Zhu Jian-bo, Effect of strain separated parts, solid-state substrates and light condition on yield and bioactive compounds ofCordyceps militarisfruiting bodies, CyTA - Journal of Food, 16, 1, 2018. Crossref
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Tang Hongbiao, Chen Chuxin, Zou Yuan, Lou Haiwei, Zheng Qianwang, Guo Liqiong, Lin Junfang, Ye Zhiwei, Yun Fan, Purification and structural characterization of a novel natural pigment: cordycepene from edible and medicinal mushroom Cordyceps militaris, Applied Microbiology and Biotechnology, 103, 19, 2019. Crossref
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Chen Xiao, Zhang Zheng, Liu Xiaoxia, Cui Bo, Miao Wentao, Cheng Weiwei, Zhao Fengyun, Characteristics Analysis Reveals the Progress of Volvariella volvacea Mycelium Subculture Degeneration, Frontiers in Microbiology, 10, 2019. Crossref
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Lou Haiwei, Lin Junfang, Guo Liqiong, Wang Xinwei, Tian Shuangqi, Liu Chenxi, Zhao Yu, Zhao Renyong, Advances in research on Cordyceps militaris degeneration, Applied Microbiology and Biotechnology, 103, 19, 2019. Crossref
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Wang Yulong, Shao Ying, Zhu Yunlan, Wang Kun, Ma Bin, Zhou Qiumei, Chen Anhui, Chen Hongwei, XRN1‐associated long non‐coding RNAs may contribute to fungal virulence and sexual development in entomopathogenic fungus Cordyceps militaris , Pest Management Science, 75, 12, 2019. Crossref
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Shao Ying, Tang Jin, Chen Shanglong, Wu Yonghua, Wang Kun, Ma Bin, Zhou Qiumei, Chen Anhui, Wang Yulong, milR4 and milR16 Mediated Fruiting Body Development in the Medicinal Fungus Cordyceps militaris, Frontiers in Microbiology, 10, 2019. Crossref
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Zhu Weiwei, Hu Jinbo, Chi Jingliang, Li Yang, Yang Bing, Hu Wenli, Chen Fei, Xu Chong, Chai Linshan, Bao Yongming, Label-Free Proteomics Reveals the Molecular Mechanism of Subculture Induced Strain Degeneration and Discovery of Indicative Index for Degeneration in Pleurotus ostreatus, Molecules, 25, 21, 2020. Crossref
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Lin Pei-Jie, Ye Zhi-Wei, Wei Tao, Wu Jia-Yue, Zheng Qian-Wang, Chen Bai-Xiong, Guo Li-Qiong, Lin Jun-Fang, Cross breeding of novel Cordyceps militaris strains with high contents of cordycepin and carotenoid by using MAT genes as selectable markers, Scientia Horticulturae, 290, 2021. Crossref
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Kontogiannatos Dimitrios, Koutrotsios Georgios, Xekalaki Savvina, Zervakis Georgios I., Biomass and Cordycepin Production by the Medicinal Mushroom Cordyceps militaris—A Review of Various Aspects and Recent Trends towards the Exploitation of a Valuable Fungus, Journal of Fungi, 7, 11, 2021. Crossref
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Zhao Fengyun, Liu Xiaoxia, Chen Chao, Cheng Zhihong, Wang Wenpei, Yun Jianmin, Successive mycelial subculturing decreased lignocellulase activity and increased ROS accumulation in Volvariella volvacea, Frontiers in Microbiology, 13, 2022. Crossref