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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019033291
pages 1-13

The Birch Bracket Medicinal Mushroom, Fomitopsis betulina (Agaricomycetes) - Bioactive Source for Beta-Glucan Fraction with Tumor Cell Migration Blocking Ability

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany
Carolin Roth
Competence Centre for Applied Mycology and Environmental Studies, Niederrhein University of Applied Sciences, Rheydter Str. 277, 41065 Mönchengladbach, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany; Department of Biomedical Sciences, Institute of Health Research and Education, University of Osnabrück, Barbarastr. 22c/ Building 93, 49076 Osnabrück, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany


Wild-grown fruiting bodies of the basidiomycete Fomitopsis betulina (Agaricomycetes, birch bracket mushroom, = Piptoporus betulinus) in different growing stages were collected and analyzed for their beta-glucan content. It could be shown that no significant difference in beta-glucan content regarding size or location of the collected fruiting bodies could be determined, but all samples displayed high values of beta-glucan in comparison to other well-known culinary or medicinal mushroom species. Furthermore, F. betulina fruiting bodies extracted with cold sodium chloride were separated into several fractions by cross flow ultrafiltration, and glucan and protein content were analyzed. The fractions showed varying amounts of beta-glucan and very low protein contents were detected. Also, bioactivity of the fractionated extract was analyzed. None of the mushroom extract fractions induced significant cytotoxicity after 48 h of incubation at a concentration up to 1 mg/mL. Interestingly, in a scratch wound assay, the extract FbS 1, an ultrafiltrated fraction > 300 kDa, was able to block tumor cell migration by 38% compared to solvent control after 48 h of incubation at a concentration of 0.33 mg/mL. In conclusion, our results have high potential for identifying novel antitumor activities based on F. betulina.


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