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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019033291
pages 1-13

The Birch Bracket Medicinal Mushroom, Fomitopsis betulina (Agaricomycetes) - Bioactive Source for Beta-Glucan Fraction with Tumor Cell Migration Blocking Ability

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany
Carolin Roth
Competence Centre for Applied Mycology and Environmental Studies, Niederrhein University of Applied Sciences, Rheydter Str. 277, 41065 Mönchengladbach, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany; Department of Biomedical Sciences, Institute of Health Research and Education, University of Osnabrück, Barbarastr. 22c/ Building 93, 49076 Osnabrück, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany

RESUMO

Wild-grown fruiting bodies of the basidiomycete Fomitopsis betulina (Agaricomycetes, birch bracket mushroom, = Piptoporus betulinus) in different growing stages were collected and analyzed for their beta-glucan content. It could be shown that no significant difference in beta-glucan content regarding size or location of the collected fruiting bodies could be determined, but all samples displayed high values of beta-glucan in comparison to other well-known culinary or medicinal mushroom species. Furthermore, F. betulina fruiting bodies extracted with cold sodium chloride were separated into several fractions by cross flow ultrafiltration, and glucan and protein content were analyzed. The fractions showed varying amounts of beta-glucan and very low protein contents were detected. Also, bioactivity of the fractionated extract was analyzed. None of the mushroom extract fractions induced significant cytotoxicity after 48 h of incubation at a concentration up to 1 mg/mL. Interestingly, in a scratch wound assay, the extract FbS 1, an ultrafiltrated fraction > 300 kDa, was able to block tumor cell migration by 38% compared to solvent control after 48 h of incubation at a concentration of 0.33 mg/mL. In conclusion, our results have high potential for identifying novel antitumor activities based on F. betulina.

Referências

  1. Kim KM, Yoon YG, Jung HS. Evaluation of the monophyly of Fomitopsis using parsimony and MCMC methods. Mycologia. 2005;97:812-22.

  2. Pleszczynska M, Lemieszek MK, Siwulski M, Wiater A, Rzeski W, Szezodrak J. Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman's polypore fungus with modern biotechnological potential. World J Microbiol Biotechnol. 2017;33:83.

  3. Lemieszek MK, Langner E, Kaczor J, Kandefer-Szersen M, Sanecka B, Mazurkiewicz W, Rzeski W. Anticancer effect of fraction isolated from medicinal birch polypore mushroom, Piptoporus betulinus (Bull.: Fr.) P. Karst (Aphyllophoromycetidadae): in vitro studies. Int J Med Mushrooms. 2009;11:351-64.

  4. Stamets P. Growing gourmet and medicinal mushrooms. 3rd ed. Berkeley, CA: Ten Speed Press; 2000.

  5. Grienke U, Zoll M, Peintner U, Rollinger JM. European medicinal polypores-a modern view on traditional uses. J Ethopharmacol. 2014;154:564-83.

  6. De Silva DD, Rapior S, Sudarman E, Stadler M, Xu J, Alias SA. Bioactive metabolites from macrofungi: ethnopharmacology, biological activities and chemistry. Fungal Divers. 2013;62:1-40.

  7. Villares A, Mateo-Vivaracho L, Guillamon E. Structural features and health properties of polysaccharides occurring in mushrooms. Agriculture. 2012;2:452-71.

  8. Zhang M, Cui SW, Cheung PCK, Wang Q. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol. 2007;18:4-19.

  9. Wasser SP. Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms. 2010;12(1):1-16.

  10. Lindequist U. The merit ofmedicinal mushrooms from a pharmaceutical point of view. Int J Med Mushrooms. 2013;15:517-23.

  11. Sari M, Prange A, Lelley J, Hambitzer R. Screening of beta-glucan contents in commercially cultivated and wild growing mushrooms. Food Chem. 2017;216:45-51.

  12. Alquini G, Carbonero ER, Rosado FR, Cosentigo C, Iacomini M. Polysaccharide from the fruiting bodies of the basidiomycete Laetiporus sulphereus (Bull.:Fr.) Murr. Microbiol Lett. 2004;230:47-52.

  13. Olennikov DN, Agafonova SV, Rokhin TA, Brovskii GB. Branched glucan from the fruiting bodies of Piptoporus betulinus (Bull.:Fr) Karst. Appl Biochem Microbiol. 2011;48:74-80.

  14. Poder R. The ice man's fungi: facts and mysteries. Int J Med Mushrooms. 2005;7:357-9.

  15. Vunduk J, Klaus A, Kozarski M, Petrovic P, Zizak Z, Niksic M, Van Griensven LJLD. Did the Iceman know better? Screening of the medicinal properties of the birch polypore medicinal mushroom, Piptoporus betulinus (higher Basidiomycetes). Int J Med Mushrooms. 2015;17:1113-25.

  16. Cyranka M, Graz M, Kaczor J, Kandefer-Szerszen M, Walczak K, Kapka-Skzypczak L, Rzeski W. Investigation of antiproliferative effects of ether and ethanol extracts of birch polypore medicinal mushroom Piptoporus betulinus (Bull.: Fr.) P. Karst. (higher Basidiomycetes) in vitro grown mycelium. Int J Med Mushrooms. 2011;13:525-33.

  17. Peintner U, Poder R. Ethnomycological remarks on the Ice-man's fungi. In: Bortenschlager S, Oeggl K, editors. The Ice man and his natural environment. Vienna: Springer; 2000, p. 143-50.

  18. Dresch P, Nives D'Aguanno M, Rosam K, Grienke U, Rollinger JM, Peintner U. Fungal strain matters: colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus. AMB Express. 2015;5:1-14.

  19. Papp N, Rudolf K, Bencsik T, Czegenyi D. Ethnomycological use of Fomes fomentarius (L.) and Piptoporus betulinus (Bull.) P. Karst. in Transylvania, Romania. Genet Res Crop Evol. 2015;64:101-11.

  20. Schlegel B, Luhmann U, Hartl A, Grafe U. Piptamine, a new antibiotic produced by Piptoporus betulinus Lu 9-1. J Antibiot. 2000;53:973-4.

  21. Grunewald F, Steinborn C, Huber R, Wille R, Meier S, Alresy Z, Lindequist U, Grundemann C. Effects of birch polypore mushroom, Piptoporus betulinus (Agaricomycetes), the "Iceman's fungus," on human immune cells. Int J Med Mushrooms. 2018;20:1135-47.

  22. Amaral AE, Carbonero ER, Simao RCG, Kadowski MK, Sassaki GL, Osaku CE. An unusual water-soluble B-glucan from the basidiocarp of the fungus Ganoderma resinaceum. Carbohydr Polym. 2008;72:473-8.

  23. El Enshasy HA, Hatti-Kaul R. Mushroom immunomodulators: unique molecules with unlimited applications. Trends Biotechnol. 2013;31:668-77.

  24. Sari M, Hambitzer R, Lelley J, Toepler K, Teusch N, Nickisch-Hartfiel A. Characterization of cross-flow ultrafiltration fractions from Grifola frondosa reveals distinct cytotoxicity in tumor cells. Int J Med Mushrooms. 2016;18:671-80.

  25. Krupodorova TA, Barshteyn VY. Alternative substrates for higher mushrooms mycelia cultivation. J Biosci Biotech. 2015;1:339-47.

  26. Gminder A. Mushrooms of Britain and Europe (Blacks's edition). London: A&C Black; 2008.

  27. Megazyme International Ireland Ltd. Mushroom and yeast B-glucan assay procedure booklet. K-YBGL 12/16. Wicklow, Ireland: Megazyme International Ireland Ltd.; 2016.

  28. Bradford MA. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1956;72:248-52.

  29. Grundemann C, Garcia-Kaufer M, Sauer B, Scheer R, Merdivan S, Bettin P, Huber R, Lindequist U. Comparative chemical and biological investigations of B-glucan-containing products from Shiitake mushrooms. J Funct Foods. 2015;18:692-702.

  30. McCleary BV, Draga A. Measurement of B-glucan in mushrooms and mycelial products. J AOAC Int. 2016;9:364-73.

  31. Ye X, Yi J, Yu R, Ru-Gang Z. Gradiation of polysaccharides using the combined method of microfiltration, ultrafiltration and nanofiltration. Food Sci Technol. 2006;8:107-10.

  32. Du X, Zhang J, Yang Y, Tang Q, Jia W, Pan W. Purification, chemical modification and immunostimulating activity of poly-saccharides from Tremella aurantialba fruit bodies. J Zhejiang Univ, Biomed Biotechnol. 2010;11:437-42.

  33. Jelsma J, Kreger D. Observations on the cell-wall compositions of the bracket fungi Laetiporus sulphureus and Piptoporus betulinus. Arch Microbiol. 1978;119(3):249-55.


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