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International Journal on Algae
SJR: 0.216 SNIP: 0.322 CiteScore™: 0.4

ISSN Imprimir: 1521-9429
ISSN On-line: 1940-4328

International Journal on Algae

DOI: 10.1615/InterJAlgae.v22.i1.50
pages 55-68

Preliminary Adaptation of Dunaliella viridis Strains to Copper Sulfate Affects the Thermal Stability of the Culture

A. I. Bozhkov
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine
M. K. Kovaleva
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine
A. V. Goltvyanskiy
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine
E. O. Ushakova
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine
H. Ye. Tsapko
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine
A. O. Gavrish
Research Institute of Biology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine


We studied the growth of copper-sensitive (CuS D. ν.) and copper-resistant (CuR D. ν. 75) strains of the green microalga Dunaliella viridis Teodoresco at 35°C to determine the relationship between the induced resistance to copper ions and resistance to a high temperature environment. The effect of stepwise temperature increasing from 24→ 29→ 35°C with an interval of 7 days on the growth rate and biomass composition (content of DNA, RNA, protein, triacylglycerides (TG), carotenoids and chlorophyll) of CuS D. ν. and CuR D. ν. cultures was examined. It was revealed that a temperature increase of up to 35°C in the culture of CuS D. ν. at the initial stage of growth slows its growth; the culture CuR D. ν. 75 dies under the same conditions. With a stepwise increase in the temperature of cultivation (24 → 29 → 35°C), the culture CuR D. ν. 75 survives, its growth rate is slightly higher than in CuS D. ν. revealing the thermal stability of its cells. In addition, biomass of CuR D. ν. 75 contains more protein, DNA, TG, and especially β-carotene, compared to CuS D. ν. At a temperature of 35°C, the content of protein, DNA, TG, and β-carotene in cells of CuS D. ν. also increased. It has been found that there is a complex relationship between resistance to copper ions and resistance to high temperature, which is determined by the temporal nature of the temperature change.


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