RT Journal Article ID 061ea0464a8f4d09 A1 Lee, Soon-Young A1 Park, Dae-Hun A1 Kim, Ji-Young A1 Lee, Young-Ju A1 Oh, Deuk-Sil A1 Bang, Mi-Ae A1 Seo, Joung-Wook T1 Genotoxicity and Single-Treatment Toxicity Evaluation of Mycoleptodonoides aitchisonii (Agaricomycetes) Water Extract JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-10-16 VO 19 IS 8 SP 717 OP 726 K1 genotoxicity K1 medicinal mushrooms K1 Mycoleptodonoides aitchisonii K1 safety K1 single-treatment toxicity, water extract AB Mushrooms have long been used worldwide for culinary and medicinal purposes because of their various nutrients and active constituents. The safety of mushrooms as a culinary ingredient requires validation. Although Mycoleptodonoides aitchisonii has long been used for culinary purposes in East Asia, it has not been authorized by a regulatory agency. In this study we conducted genotoxicity and single-treatment toxicity tests according to the guidelines of the Organisation for Economic Co-operation and Development. We performed genotoxicity tests (bacterial reverse mutation study, chromosome aberration test, and micronucleus test), single-treatment toxicity tests, and in vivo mammalian alkaline comet assay of M. aitchisonii water extract (WT). A single treatment with 5000 mg/kg M. aitchisonii WT showed no toxicity. M. aitchisonii WT induced bacterial reverse mutation and chromosome aberration but showed a negative result in the micronucleus test. Thus, an in vivo mammalian alkaline comet assay was performed; however, no genotoxicity was detected. Treatment with <5000 mg/kg M. aitchisonii WT is nontoxic and can be used for culinary purposes. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,32c8651274405055,061ea0464a8f4d09.html