%0 Journal Article %A Brugnari, Tatiane %A da Silva, Pedro Henrique Alves %A Contato, Alex Graça %A Inácio, Fabíola Dorneles %A Nolli, Mariene Marques %A Kato, Camila Gabriel %A Peralta, Rosane Marina %A de Souza, Cristina Giatti Marques %D 2018 %I Begell House %K antimicrobial, antioxidant activity, cooking, cytotoxicity, in vitro digestion, medicinal mushrooms, Pleurotus ostreatoroseus %N 3 %P 259-270 %R 10.1615/IntJMedMushrooms.2018025815 %T Effects of Cooking and In Vitro Digestion on Antioxidant Properties and Cytotoxicity of the Culinary-Medicinal Mushroom Pleurotus ostreatoroseus (Agaricomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,08829abe31900427,2d7ecd1d7d66dc80.html %V 20 %X In this study we evaluated the antioxidant capacity, antimicrobial activity, and cytotoxicity of an aqueous extract of the Pleurotus ostreatoroseus mushroom, which was cooked. Fresh basidiocarps were heated and steamed at 100°C and the resulting aqueous extract was assessed before and after in vitro digestion. Cooking reduced the amounts of phenolic compounds in the extract. The antioxidant activity of the extract was evaluated through the use of 4 methods. The lowest half-maximal effective concentration (EC50) against ABTS radicals was 0.057 ± 0.002 mg/mL for the uncooked basidiocarp extract. Cooking and the digestive process led to decreased activity (P > 0.05) against ABTS and DPPH radicals. A significant increase in chelating activity (P > 0.05) occurred after the basidiocarps were cooked (EC50 = 0.279 ± 0.007 mg/mL). The reducing power did not significantly change among the different extracts. The uncooked basidiocarp extract was cytotoxic to Vero cells. After cooking and subsequent in vitro digestion, the cytotoxicity of the extracts decreased (P < 0.05). Bacillus subtilis, Staphylococcus aureus, and Candida albicans were sensitive to the fresh mushroom extract. The data showed that after being cooked and digested, the P. ostreatoroseus mushroom maintains antioxidant activity and has a low cytotoxic effect. %8 2018-04-23