RT Journal Article ID 32a9d6f975836f13 A1 Davitashvili, Elene A1 Kapanadze, Ekaterine A1 Kachlishvili, Eva A1 Mikiashvili, Nona A. A1 Elisashvili, Vladimir I. T1 Isolation and Characterization of Lectins Formed by Cerrena unicolor (Higher Basidiomycetes) in Solid-State Fermentation of Sorghum and Wheat Straw JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-06-17 VO 17 IS 5 SP 427 OP 434 K1 medicinal mushrooms K1 Cerrena unicolor K1 galactose-specific lectins K1 mycelium biomass K1 primordia K1 SSF AB The capability of Cerrena unicolor to produce fruiting bodies and lectins was studied in solid-state fermentation of a sorghum and wheat straw mixture. The first primordia appeared on day 48 and reached 6−10 mm; however, no formation of fruiting bodies occurred and these rudiments were harvested on day 55. The protein content in the rudiment extracts was significantly higher, whereas the specific hemagglutinating activity (HA) was sixfold lower as compared with those in extracts from mycelial biomass. Moreover, the specific HA of the 80-day mycelium increased to 16,667 U/mg, exceeding by sixfold that of 55-day-old mycelium. Four protein fractions (160, 105, 67, and 8 kDa) were detected by gel-chromatography of mycelial biomass crude extract; the highest specific HA was revealed in fraction III (26336 U HA/mg). Among sugars tested, galactose was the most potent inhibitor of HA of all protein fractions, with minimal inhibition concentrations of 0.095−0.780 mM. The galactose-specific lectins isolated from the fractions II and III by affinity chromatography ranged from 15 to 116 kDa and differed with kinetic parameters. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,118fb2a34500f533,32a9d6f975836f13.html