RT Journal Article
ID 375bdb096256199a
A1 Cohen-Hadar, Noa
A1 Wine, Yariv
A1 Lagziel-Simis, Shira
A1 Moscovich-Dagan, Hila
A1 Dror, Yael
A1 Frolow, Felix
A1 Freeman, Amihay
T1 Protein Crystal-Mediated Biotemplating
JF Journal of Porous Media
JO JPM
YR 2009
FD 2008-10-24
VO 12
IS 3
SP 213
OP 220
AB Protein crystals, routinely prepared for the elucidation of protein 3-D structure by X-ray crystallography, present a highly accurate 3-D array of protein molecules. Along with the formation of this array, a complementary 3-D array of voids is also formed, with pattern, geometry, and size depending on the protein “building-block” and its packaging within the crystal. Here we present recent results demonstrating the feasibility of using the pores of protein crystals as a biotemplate for the preparation of composite materials. Our previously developed methods of monitoring the preservation of the 3-D protein crystal template throughout biotemplating of chemically cross-linked hydrogels and the elucidation of the mechanism of protein crystal cross-linking by glutaraldehyde were applied in this study to enable and demonstrate the feasibility of protein crystal-mediated biotemplating resulting in a 3-D array of silver particles located within the pores of the crystal. Our accumulated results indicate that the pool of protein crystals may be successfully exploited as a source of biotemplates for the fabrication of novel composite materials, in contrast to the common consideration of protein crystals as labile structures, useful only for the elucidation of the 3-D structure of proteins.
PB Begell House
LK https://www.dl.begellhouse.com/journals/49dcde6d4c0809db,3de92af770e695c5,375bdb096256199a.html