RT Journal Article ID 38e8285f7b4e4ba2 A1 Xu, Xin A1 Pang, Chong A1 Yang, Chengjian A1 Zheng, Yitong A1 Xu, Hong-Yu A1 Lu, Zhenming A1 Xu, Zheng-Hong T1 Antihyperglycemic and Antilipidperoxidative Effects of Polysaccharides Extracted from Medicinal Mushroom Chaga, Inonotus obliquus (Pers.: Fr.) Pilát (Aphyllophoromycetideae) on Alloxan-Diabetes Mice JF International Journal of Medicinal Mushrooms JO IJM YR 2010 FD 2010-08-24 VO 12 IS 3 SP 235 OP 244 K1 medicinal mushrooms K1 polysaccharides K1 antihyperglycemic K1 antilipidperoxidative K1 antioxidant K1 diabetes K1 chaga mushroom K1 Inonotus obliquus AB The optimum conditions for the extraction of crude polysaccharides from dry matter of culture broth (DMCB) of Inonotus obliquus were determined by using response surface methodology (RSM). In alloxan-induced diabetic mice, treatment with polysaccharide extract from DMCB of I. obliquus (150 and 300 mg/kg body weight for 21 days) showed a significant decrease in blood glucose level, with percentage reductions of 16.64% and 20.09% at the 7th day, and 29.71% and 36.36% at the 21st day, respectively. Furthermore, polysaccharide treatment significantly decreased serum contents of free fatty acid, total cholesterol, triglyceride, and low-density lipoprotein cholesterol, whereas it effectively increased high-density lipoprotein cholesterol, insulin levels, and hepatic glycogen contents in the liver of diabetic mice. In addition, the polysaccharides significantly increased catalase, superoxide dismutase, and glutathione peroxidase activities, whereas they decreased the maleic dialdehyde level in diabetic mice. A histological morphology examination showed that the polysaccharides restored the damage of pancreatic tissues in mice with diabetes mellitus. The results revealed that the I. obliquus polysaccharides possessed antihyperglycemic, antilipidperoxidative, and antioxidant effects in alloxan-induced diabetic mice. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2da9c44d295f30f8,38e8285f7b4e4ba2.html